Apelin Inhibit H9c2 Cardiomyocytes Hypertrophy Induced By Resistin

Objective:Through apelin interference H9c2 cardiomyocyte hypertrophy model induced by resistin, determine the surface area size of H9c2 cardiomyocytes, the total protein synthesis quantity of single cell, and the expression amount of cardiomyocyte hypertrophy markers BNP and β-MHC by RT-q PCR technology, study the role of apelin to the H9c2 cardiomyocytes hypertrophy induced by resistin, explore the new method of effective prevention and treatment of cardiomyocyte hypertrophy, and applied to provide theoretical basis for the prevention and treatment of heart failure.Methods:H9c2 rat cardiomyocytes were cloned cell lines, which came from BD1 X rat embryonic heart tissue, culturing with DMEM containing 10% FBS, replacing new nutrient solution every 2 or3 days. Stay subculture when cells grown to 70%-80%.H9c2 rat cardiomyocytes were the circular or elliptic observed under inverted microscope, no heart throb. 1×105H9c2 rat cardiomyocytes were inoculated in the culture plate, culturing with 3 ml DMEM containing 10% FBS, after being adherent,replace 3 ml DMEM containing 0.1% FBS for hungering 16 hours. Experiment was divided into four groups according to the different experimental protocol. Group C:The control group. After cell hunger replaced with 3 ml DMEM containing 0.1% FBS culture for 48 hours. Group R: The resistin group. After cell hunger replaced with 3ml resistin concentration of 50 ng/ml DMEM containing 0.1% FBS culture for 48 h.Group A: The apelin + resistin group. After cell hunger replaced with 3 ml apelin concentration of 10 μmol/3 ml DMEM containing 0.1% FBS cultureing 2 h, and then replaced with 3 ml resistin concentration of 50 ng/ml DMEM containing 0.1% FBS culture for 46 h. Group N: LKB1 inhibitor + Apelin + resistin group. After cell hunger replaced with 3 ml LKB1 inhibitor(HNE) concentration for 20 μmol/3 ml DMEM containing 0.1% FBS, 2 hours later replace 3 ml apelin concentration of 10μmol/3 ml DMEM containing 0.1% FBS cultureing 30 minutes, and then replaced with 3 ml resistin concentration of 50 ng/ml DMEM containing 0.1% FBS culturedfor 45.5 h. After 48 h, the surface area size of H9c2 cardiomyocytes and the total protein synthesis quantity of single cell detected, and the expression amount of cardiomyocytes hypertrophy markers BNP and β-MHC were also detected by RT-q PCR technology.Results:Observing the surface area size of H9c2 cardiomyocytes, the total protein synthesis quantity of single cell, and the expression amount of cardiomyocytes hypertrophy markers BNP and β-MHC, the group C compared with the group R was statistically significant(P < 0.01), resistin induced H9c2 cardiomyocytes hypertrophy model was successfully built.The group R compared with the group A was statistically significant(P < 0.01). There was no statistically significant difference between group R and group N(P > 0.05).Conclusions:Apelin can inhibit the H9c2 cardiomyocyte hypertrophy induced by resistin. The underly mechanism may be involving LKB1/AMPK pathway.