Effect Of FRNK On Apoptosis In Keloid Fibroblasts

Objective To evaluate the effect of FRNK on apoptosis in keloid fibroblasts,and the relationship along the inhibitory effect of FRNK on the phosphorylation of FAK and the signaling pathway of FAK-ERK,and the expression of COL1A1 and COL3A1 in keloid fibroblasts. Methods The expression of FRNK in the keloid tissues and the normal skin was detected by western blotting and RT-PCR.FRNK plasmid mediated by cationic liposome was transfected into keloid fibroblasts in vitro.The apoptesis of FRNK-induced keloid fibroblasts was examined by Annexin-V/propidium iodide double-labeled flow cytometry(FCM).The protein levels of FRNK,FAK, P-FAK,ERK and p-ERK in keloid fibroblasts were assayed by Western blottingting.The level of FAK,ERK,COL1A1,COL3A1 were assayed by RT-PCR. Results â‘ The expression of FRNK was significiant different between the keloid tissues with the normal skin tissues(P<0.05).â‘¡The up-regulated expression of FRNK protein was observed and it was at 48 hour after transfection that the FRNK protein content was the highest(P<0.05),and down-regulated at 72 hour.â‘¢Compared with the n FRNK plasmid group,the expression of FAK,p-FAK,ERK,p-ERK,COL1A1 and COL3A1 in FRNK plasmid group reduced(P<0.05).â‘£The apoptotic rate in keloid fibroblasts expose to FRNK plasmid was higher than that in the n FBNK plasmid group(P<0.05). Conclusions â‘ The expression of FRNK protein in keloid promotes the apotosis of fibroblast.â‘¡After FRNK is transfected successfully by using Lipofectamine, FRNK induces the apoptosis of keloid fibroblasts by inhibiting the phosphoryation of FAK and ERK,and down-regulated the FAK-ERK signal transduction pathway.â‘¢FRNK induces the apoptosis of keloid fibroblasts may results in the regradation of collagen.