Influence Of Percutaneous Stimulation Of Hepatic Region With Mid-Frequency Pulse Current On The Central Neural System Fatigue In Rats

ObjectiveTo observe the effect of the percutaneous stimulation of hepatic region with mid-frequency pulse current on the anti-fatigue ability and the level of free radicals and nissl bodies in cerebral cortex tissue, and the concentration of 5-hydroxytryptamine(5-HT) and its metabolism in striatum of fatigued rats, and explore the effects of percutaneous stimulation of hepatic region with mid-frequency pulse current on the central neural system fatigue in rats, then provide experimental evidence and theoretical support for the clinical application of its treatment of central neural system fatigue. MethodsEight-week-year old Wistar male rats were randomly separated into four groups: control group(CG, n=18), fatigue group(FG, n=18), stimulation before fatigue group(SBF, n=18) and stimulation after fatigue group(SAF, n=18). All of the groups were given the swimming training to establish exercise-induced fatigue model except the CG group. Percutaneous stimulation of hepatic region with mid-frequency pulse current were given on the SBF group before swimming and the SAF group After exhaustive swimming(frequency was1024 Hz, current intensity was 10 m A, period was 1s and the lasting time was 20min). At the last day of 1st, 3rd, and 5th week after modeling, rats’ exhaustive swimming time of each group was observed except the CG group. Cerebral cortex tissue was dissected to measure the level of lipid peroxidation, such as SOD, MDA, GSH-Px and SOD/MDA, and the histopathological changes of nissl bodies in neurons was observed. And the level of free-tryptophan(F-Trp), branched chain amino aci(BCAA) and the ratios of F-Trp/ BCAA were measured in blood serum of rats, the concentration of F-Trp, 5-HT and 5-hydroxyindoleacetic acid(5-HIAA) were tested in Striatum of Rats. Result1. At the weekend of 1st week, the indexes were not statistically significant. At the weekend of 3rd week, the exhaustive time of SAF group was obviously longer than that of the FG group, and the SAF group was obviously longer than that of the FG group and SBF group at the weekend of 5th week(P<0.05). At the weekend of 5th week, as to the SOD、GSH-Px and SOD/MDA contents, the FG group and the SBF group were obviously less than that of the CG group and SAF group, and the MDA content were obviously more than that of the CG group and SAF group(P<0.05). And as to the nissle bodies in neurons, it is observed that the ratio of number and area of the SAF group was obviously higher than that of FG group and SBF group at the weekend of 5th week(P<0.01).2. Compared with CG group, the concentrations of BCAA in blood serum of rats in every group tended to decline, other indexes were all increased. Compared with FG group, the concentrations of 5-HT in rats’ striatum in SAF group and SBF group declined significantly(P<0.01) at the weekend of 3rd week. Compared with SBF group, the concentrations of 5-HT in rats’ striatum in SAF group declined significantly(P<0.01) at the weekend of 5th week. Conclusion1. Percutaneous stimulation of hepatic region with mid-frequency pulse current can effectively lessen the lipid peroxidation damage of cerebral cortex tissue and lessen the dissolution and disappearance of nissl bodies in fatigued rats, increase exhaustive exercise time and postpone fatigue.2. Percutaneous stimulation of hepatic region with mid-frequency pulse current can effectively lessen the concentration of 5-HT and its metabolism in fatigued rats, which could suspend the occurrence of fatigue and help to eliminate the fatigue,improve rat central nerves performance in long time exhausted exercise.