The Signal Crosstalk About STAT3 Pathway Between Glioma Cells And The Human Brain Microvascular Endothelial Cells

Glioma is the most common malignant tumor of the central nervous system, which has the characteristics of low mortality rate and high cure rate. According To Statistics, The median surial of patients with malignant glioma is only 17.9 months. Lateral ventricle gliomas patient’s prognosis is worse, who can only survive by 5.7 to 17.5 months. Glioma is a complex process which is more than a factor of participation, more genetic changes and multi-step evolution. The reason of glioma patients with poor prognosis associated with glioma’s biological behavior.Accordingly, researching the malignant behavior and related molecular mechanism of glioma has important implications. Most of the research were stay on signal transmission of internal tumor cells and the impact of the change of the nutritional components of tumor microenvironment. Thus they ignored the microvascular endothelial cells which were the important components in the microenvironment. The microvascular endothelial cells play an critical role in the process of proliferation and invasion of tumor cells. Therefore, Studying the interaction between microvascular endothelial cells and tumor cells has theoretical and practical significance.As one of the most researched tumor signal pathway, IL-6/STAT3 pathway perhaps plays a role as a bridge in this process. So this study choosed this pathway to research the crosstalk between the glioma cells(A172/U251) and the Human brain microvascular endothelial cells.This research used fllowing method to testify the signal Crosstalk about STAT3 pathway between glioma cells and the Human brain microvascular endothelial cells.Elisa was performed to detect the secretion of the IL-6 of endothelial cells which was coultured with VEGF、 glioma cells for 24 h, 48 h, 72 h. Western blotting was performed to investigate the change of STAT3 protein and its phosphorylation status of the glioma cells after cultured with IL-6(50 ng.ml-1), endothelial cells and endothelial cells+AZD1480.CCK-8 assay was used to detect the effect of interleukin6(50 ng.ml-1), endothelial cells and endothelial cells+AZD1480 on proliferation of glioma cells, and the relative proliferation effect was calculated. Transwell assay was conducted to study the effect of IL-6(50 ng.ml-1), endothelial cells and(endothelial cells+AZD1480) on invasion of glioma cells. The main experimental results are as follows: Results:1. Glioma cells and VEGF1 6 5 promoted the secretion of the IL-6 of endothelial cells(P<0.01).2. IL-6(50 ng.ml-1) and endothelial cells enhanced the expression of the P-STAT3 protein(P<0.01). AZD1480 inhibit the expression of the P-STAT3 protein.3. Interleukin6(50ng/ml) and endothelial cells enhanced the growth and invasion of the glioma cells(P<0.01). AZD1480 suppressed the growth and invasion of the glioma cells(P<0.01). Conclusions:1. The Human brain microvascular endothelial cells and its secretion factor can significantly promote the level of proliferation and invasion ability of glioma cell.2. The Human brain microvascular endothelial cells and its secretion factor can significantly promote the expression of the P-STAT3 protein.3. Glioma cells and their secretory factor VEGF can promote the Human brain microvascular endothelial cells secrete cytokine interleukin- 6.