The Study On Analgesic Effects Of Oxymatrine Involved In NF-κB Pathway On Neuropathic Pain

Objectives We investigated the antinociceptive effects of Oxymatrine(OMT) via adjust of NF-κ B signal pathway in a chronic neuropathic pain model induced by chronic constrictive injury(CCI) of the sciatic nerve.Methods 1. The antinociceptive effects of OMT on neuropathic pain were evaluated by using von frey filaments and heat radiants tests.2. Immunofluorescence, Q-PCR and western blotting assay were performed to assess the expressions of NF-κ B immuno-staining positive neurons, NF-κ B, TNF-α, IL-6, IL-1β and IL-10 m RNA and protein.Results 1. The intraperitoneal administration of OMT(160 and 80 mg/kg) significantly increase the paw withdrawal threshold(P<0.05), and enhance the paw withdrawal latency(P<0.05) compared with CCI group.2. Intraperitoneal administration of OMT(160 mg/kg) remakedly reduced the expressions of NF-κ B P65 immuno-staining positive neurons and NF-κ B P65, TNF-α, IL-6and IL-1β mRNA and protein and increased the expressions of IL-10 protein(P<0.01)compared with CCI group.Conclusion OMT has antinociceptive effects on neuropathic pain induced by chronic constrictive injury and the antinociceptive effects of OMT on neuropathic pain were related with down-regulation the expressions of NF-κ B, TNF-α, IL-6, IL-1β and up-regulation the expression of IL-10.