The Expression And Clinical Significance Of PrP~C In Rectal Carcinoma

Objective:By detecting the expression of Cellular prion protein(PrPC) in rectal carcinoma tissue, rectal adenoma tissue, and corresponding normal rectal tissue adjacent to carcinoma, and analyses of the correlation between the expression of PrPC in rectal carcinoma tissue and clinical pathological parameter including tumor differentiation, clinical stages, lymph node metastasis and so on. To probe its clinical significance in rectal carcinoma occurrence and development, providing theoretical basis for elucidating the pathogenesis of rectal carcinoma. Methodology:Surgical specimens of formalin-fixed and paraffine-embedded were collected from Gastrointestinal Surgery Department of the First Affiliated Hospital of Nanchang University from June 2013 to April 2014, including 60 cases of rectal carcinoma tissue, 20 cases of the corresponding normal rectal tissue adjacent to carcinoma and 20 cases of rectal adenoma were collected as the control group. Immunohistochemical SP method was used to detect the expression of PrPC in rectal carcinoma, rectal adenoma and adjacent normal tissue, and statistical the expression rate of PrPC respectively in each group. Using statistical analysis software SPSS19.0 to analysis and disposal experimental data. Results:The positive rate of PrPC expression in rectal carcinoma(65%) was higher than that in rectal adenoma(35%) and normal rectal mucosa(15%)(P<0.05). But the positive expression of PrPC in rectal adenoma and normal rectal mucosa tissues had no significant difference(P>0.05). The positive expression ratios of PrPC in rectal carcinoma tissue divided into two groups according to the tumor differentiation were 54.76%(23/42) and 88.89%(16/18) respectively. The positive expression rate of PrPC in rectal carcinoma tissue divided into two groups on the basis of different stages of TNM classification were 45.45%(10/22) and 76.32%(29/38) respectively. The posi-tive expression rate of PrPC in rectal carcinoma tissue with lymph nodes metastasis and without lymph nodes metastasis was 77.78%(28/36) and 45.83%(11/24) respectively. The above mentioned results indicated that the positive rate of PrPC was closely related with tumor differentiation, TNM stage and lymph node metastasis(P<0.05). However, the expression of PrPC in rectal carcinoma was not related with sex, age, tumour location and tumor size(P>0.05). Conclusion:The expression of PrPC in rectal carcinoma tissue is higher than that in rectal adenoma and normal rectal mucosa, indicates that PrPC may play an important role in the occurrence and development of rectal carcinoma.The level of PrPC expression in rectal carcinoma is significantly correlated with tumor differentiation degree, TNM neoplasm staging and lymph node metastasis, which prompts PrPC may participate in the development, invasion and metastasis of rectal carcinoma.