A Study On The Effects Of Allograft Olfactory Ensheathing Tissue Transplantation Upon The Expression Of IL-1β In The Spinal Cord Of Rats With Neuropathic Pain

Background: Neuropathic pain(Neuropathic pain, NP) is a chronic neuropathic pain that directly caused by a primary or secondary damage or dysfunction of the central nervous system or peripheral nervous system. Its main feature is persistent pain, tactile allodynia pain and hyperalgesia. NP has a variety of etiology and complex pathogenesis, as its high disease rate and seriously affects the quality of patients’ life, NP has been an urgent global health problem to be solve.However, the clinical effects of drug treatment is not ideal. In order to seek better treatment, domestic and foreign scholars have conducted extensive research and found that cell transplantation may be an ideal strategy for the treatment of NP.Objective: To investigate the role of IL-1β in allogeneic olfactory ensheathing tissue transplantation for the treatment of neuropathic by observing the change of interleukin-1β(Interleukin-1β, IL-1β) m RNA and protein expression levels in spinal cord of rats with neuropathic pain which have transplantated olfactory ensheathing allograft tissue.Methods: We divided randomly healthy adult SD rats into normal group(Normal group), sham-operated group(Sham group), chronic constriction injury group(CCI group) and CCI + olfactory bulb tissue transplantation group(CCI + OBTT group), mechanical withdrawal threshold(MWT) and thermal withdrawal latency(TWL) of rats were measured before and after operation 7 days, 14 days. Then take the Lumbar 4 and 5 spinal segment of rats, we used reverse transcription polymerase chain reaction(RT-PCR) and Western blot(Western blotting) to measure IL-1β m RNA and protein expression level in spinal cord of rats after operation 7 days and 14 days.Results: In 7 days and 14 days, MWT and TWL in CCI group were significantly lower than them in Sham group(p<0.05), while MWT and TWL in CCI + OBTT group were significantly higher than them in CCI group(p<0.05). In 7 days and 14 days, the expression levels of IL-1β m RNA and protein were significantly higher in spinal cord of rats on operation side in CCI group than Sham group(p<0.05), while the expression levels of IL-1β m RNA and protein in CCI + OBTT group were significantly lower than CCI group(p<0.05), but still higher than Sham group. Compared with Normal group, MWT、TWL、the levels of IL-1β m RNA and protein in Sham group had no significant difference.Conclusion: Olfactory bulb tissue transplantation may alleviate neuropathic pain of rats with CCI by down-regulating the expression levels of IL-1β m RNA and protein in spinal cord.