Berberine’s Effect And Mechanism On The Adhesion Fuction Of Podocyte In Diabetic Nephropathy Rats.

Objective:To investigate the protective effects(The influence of the adhesion molecules) of berberine the main active component of cortex phellodendri, on the kidney and podocyte of diabetic nephropathy(DN) rat, and to explore the molecular level to interpret of diabetic nephropathy(DN) renal failure of consolidation in chinese medicine theory. Examining the BBR’s function of tonify kindey astringent therpy.Method:30 male Zucker(fa/fa) rat of SPF grade,aged 8 weeks,10 male Zucker lean(ZL) rat as negativcontrol of SPF grade were used in this study.All rats are free standard diet and wateintake(fodder call Purina#5008 will contain approximately 23.5% crude protein and 6.5% crudfat).The NG(N=10) and diabetic nephropathy(DN) group(30) include the MG(N=10),the BBgroup(N=10),the VAL group(N=10).Drug intervention started in successful DN model wainduced and lasted 8 weeks.During the therapeutic interventions period,FBG and 24 h U-Alb werobserved dynamically at 0,2,4,6,8 weeks. Rats were executed till the end of 8th week, abdominaaortic blood and took out a sample of kidney tissue,measuring blood fat, insulin, serum creatinine(Scr),blood urea nitrogen(BUN).Taken each renal cortex for testing.The proteiexpression level of adhesion molecule α3β1 integrin, integrin-linked kinase(ILK) ancytoskeletal protein alpha-actinin-4(α-actinin-4) within the renal cortex was analysed bReal-Time PCR and Western Blot, immunohistochemical techniques.Morphological observatiowas observed under electron microscope.Results:1.Compared to the MG group,the FBG、24h U-Alb、TC、TG were significantly lower in BBR group after treatment,and the insulin levels was significantly increased in BBR group after treatment;Compared to the VAL group,the FBG、24h U-Alb、TC、TG、insulin levels were no significant difference in BBR group after treatment.2.Compared to the MG group,BBR group of the α3β1 integrin’s m RNA and Protein, were significantly difference after treatment.Compared to the VAL group,BBR group of the α3β1integrin’s m RNA and Protein were no significant difference after treatment.3.Compared to the MG group, the BBR group of ILK’s m RNA and Protein were significantly difference after treatment.Compared to the VAL group, the BBR group of ILK’s m RNA and Protein were no significantly difference after treatment.4.Compared to the MG group, the BBR group of α-actinin-4’s m RNA and Protein were significantly difference after treatment.Compared to the VAL group, the BBR group ofα-actinin-4’s m RNA and Protein were no significantly difference after treatment.Conclusions:BBR has a protective effect on DN kideneys. BBR reduces blood glucose and urinary albuimin excretion, improves the adhesion of podocytes and delays the development of DN.