Effect Of Nano-hydroxyapatite Combined With PRP And BMP-4 After Core Deconmpression On Treating Femoral Head Necrosis In Rabbit

ObjectiveTo research the feasibility of Nano-Hydroxyapatite(Nano-HA) combined with platelet rich plasma(PRP) and bone morphogenetic protein-4(BMP-4) after core decompression in the treatment of rabbit femoral head necrosis.MethodsAnimal models of femoral head necrosis were made in rabbits by injecting hormone, 36 rabbits were chosen to divided into 3 groups randomly: A,B and C, and every group had 12 rabbits. Group A was untreated as the model group and did not receive any treatment; group B was treated with core decompression; group C was implanted Nano-HA combined with PRP and BMP-4 after core decompression. The treatment ability was assessed by histopathological examination and bone trabecula image analysis at 2,4,8 weeks and density measurement at 8 weeks after operation. And using SPSS software for data analysis Results(1) Histopathological examination: the osteogenic capability of group C was always better than group A and B. Bone trabecula appeared in group C in the 2th week, and in the 8th the bone trabecula was mature, bone marrow tissue formed. While in group B in the 8th week, bone necrosis was still repairing, and bone marrow tissue appeared. There was still no bone tissue in group A in the 8th week.(2) Bone trabecula image analysis: the bone trabecula proportion of group C was higher than group B in the 2th, 4th and 8th week, and the difference had statistical significance(p<0.05).(3) Density measurement: the bone density of group C was higher than group A and B in the 8th week, and the difference had statistical significance(p<0.05). ConclusionPRP combined with BMP-4 can induce new bone formation, the Nano-HA can be a good scaffold material for repairing femoral head necrosis after core decompression, and Nano-HA combined with PRP and BMP-4 after core decompression can accelerate repairing femoral head necrosis and has a very promising applicable future.