Delaying Of Denervated Skeletal Muscle Atrophy In Rats By Means Of Interfering MuRF1 By RNAi

Objective:Explore the lentivirus transfection RNAi technology mediated Mu RF1 genes were slow loss of nerve the curative effect of skeletal muscle atrophy in rats. For clinical nerve after the study and treatment of skeletal muscle atrophy experimental basis and provide the reference.Methods:Age 6 to 8 weeks SD rats, take on an empty stomach weight for 170-190 g of 40 rats,were randomly divided into experimental group a total of 20(50 mu L injection restructuring slow virus load Lenti, GFP- Mu RF1) and control group of 20(injection Lenti. 50 mu L GFP solution). Cut off the right hip sciatic nerve of rats in both groups,set up right foot long extensor neural muscle atrophy. Model experiment way after loss of nerve of rats foot long extensor injection of 50 mu L restructuring slow virus load Lenti, GFP- Mu RF1; Control group injected Lenti, GFP solution 50 mu L, 1, 3 weeks after the injection, every time two groups of rats were taken 6, only a complete show load-point after resection of the right foot long extensor, on fluorescence microscope green fluorescent signal, two groups of rats were taken each time points 6, complete resection of the right toe long extensor muscle cell ultrastructure observation, determine the amount of muscle protein content, muscle wet weight maintenance ratio, rt-pcr detection Mu RF1 gene, detect myocyte cross-sectional area.Results:As time to one week and three weeks after transfection, two groups of toe long extensor can be observed that a large number of GFP fluorescence signal. 1 weeks after transfection group maintain muscle wet weight, muscle cross-sectional area, long toe extensor muscle total protein content, respectively(90.87 + 4.56) %,(943.53-24.27) mu /㎡(94.18-2.53) and(mg/m L) were significantly higher than control group(75.73 +6.21) %,(753.43-23.41) mu/㎡(72.54-2.45) and(mg/m L), the difference had statistical significance(P < 0.05). Rt-pcr detection experimental Mu RF1 gene compared with the control group significantly lowered(P < 0.05). Transfection after 3 weeks, the above indexes respectively(84.37 + 6.24) %,(851.30-20.34) mu/㎡(81.37-2.84) and(mg/m L),is still higher than the control group, the index for the control group(50.42 + 5.01) %,(643.75-21.90) mu ㎡(59.72 + 3.62)(mg/m L)(P < 0.05). Ultrastructure showed that postoperative one week group degeneration of nucleus.Conclusions:Delaying of denervated skeletal muscle atrophy in rats by means of interfering Mu RF1 by RNAi...