Study On Genetic Polymorphism Of 30 Indel Lociin Fujian She Population

Objective: To explore the polymorphic distribution of 30 Indel loci included in Fujian She population. To evaluate the forensic application value of 30 insertion/Deletion(INDEL) loci included in Investigator®DIPplex system and its potential forensic application in domestic population.Methods:(1) DNA of 200 unrelated healthy individuals(male100,female100) of Han population in Fujian was randomly extracted and amplified by multiplex PCR system. Then PCR products were separated and genotyped with capillary electrophoresis and fluorescence detection technology.(2) Allele frequencies, population genetics parameters of the 30 INDEL loci were statistically calculated.(3) Compared the differences of Allele frequencies between Fujian Han population and other populations which were analyzed by reference to domestic and foreign literatures.Results:(1) In Fujian She population, the allele frequency of the 30 In Del loci was F(del): 0.133-0.853,the average was 0.506, F(ins): 0.148-0.868,the average was 0.495, the Homozygotes(Ho) was 0.45-0.82, the average was 0.598; the power of discrimination(DP) was 0.385-0.645, the average was 0.581; the polymorphism information content(PIC) was 0.2-0.37, the average was 0.338; the Random Matching Probability(RMP) was0.355-0.615, the average was 0.419; the probability of paternity exclusion was 0.025-0.235, the average was 0.131.(2) The combined power of exclusion(CPE) was 0.9862458 and the combined discrimination power(CDP) was 0.99999999999680861.(3) Fujian She population of 30 In Del loci in genotype distribution are fully consistent with the balance of the Hardy-Weinberg(P>0.05) based on the Bonferroni correction.(4) Compare of Fujian She population and Fujian Han population, the average F(del), the average F(ins), average PIC, average PE, average DP and CPE have little difference(P>0.05), but the average values observed of Ho haslarge difference, and the frequency of the alleles of HLD118、 HLD81、 HLD39 performs significant difference(P<0.05) between these two populations.(5) The average F(del) and F(ins) are basically the same(P>0.05) between She population in East China and Fujian, but the values observed of Ho which was big difference between She population in East China and Fujian were 0.635 and 0.39, respectively.(6) The F(del) frequency of the loci of HLD118、HLD99、 HLD122、HLD40、HLD39 in Fujian She population was significantly higher than that of other ethnic groups(Tibetan, Uygur and Kazak)(P<0.05).(7) Compared with Poland, USA, Germany and Africa, the F(del) distribution of the loci of HLD111(P<0.05)in Fujian She population perform higher frequency, but the HLD118(P<0.05)distribute lower frequency.Conclusions:(1) There are relatively high genetic polymorphism and some differences of the allele frequency and gene diversity of 30 In Del loci included in Fujian She population, which might contribute to promoting the study of the genetics in Fujian She population.(2) Fujian She population of 30 In Del loci in genotype distribution are fully consistent with the balance of the Hardy-Weinberg(P>0.05) based on the Bonferroni correction. The results suggest the 30 In Del loci on the same chromosome in Fujian She population are not genetic linkages.(3) At 30 In Del loci, HLD39 and HLD118 loci can be used to assist the identification of the difference between Fujian She and Tibetan, Uygur and kazak.(4) Fujian She population possess high frequency in the loci of HLD111, and low frequency in HLD118, which can be used to assist in differentiating Fujian She population and Europe, United States and Africa group.(5) CDP of 30 In Del loci included in Fujian She population meet the requirements for the individual identification, which could be applied to individual identification independently, but the CPE was lower. Investigator®DIPplex kit might be used as a valuable supplement to Fujian She parentage testing.