The Significance Of Serum Level Of S100B Protein And NSE In Children With Tic Disorders

Objective1. To study the level of serum S100 B protein and NSE in healthy children and children with tic disorder, to explore whether tic disorder exsit brain injury or not.2. To study the level of serum S100 B protein and NSE in children with tic disorder before and after treatment by aripiprazole, to explore whether the brain injury was improved after the clinical symptoms have been controlled. Methods1. We used DSM-IV about TD as inclusion criteria. The children were selected from outpatient of pediatric department in Fujian Provincial Hospital. There were 63 children in TD group and 20 healthy children in control group.2. A 4 ml venous blood samples were collected at the first visit in all cases and TD group after 12 weeks treatment. Centrifuge the blood and collect the upper serum. The serum was stored in refrigerator until the assay. Enzyme-linked immunosorbent assay was used to detect the level of serum S100 B protein and immune chemiluminescence was used to detect the level of serum NSE.3. All children in TD group accept the treatment of Aripiprazole with a duration of 12 weeks. YGTSS was used to evaluate the illness degree and the adverse reaction scale was used to evaluate the adverse reaction.4. Software SPSS 19.0 Version was used for statistics. Results1. There were no significant correlation between the concentration of serum S100 B protein and the age in healthy children and children with tic disorder.(r =-0.069, P >0.05; r=-0.003, P >0.05)2. The levels of serum S100 B protein and NSE in children of TD group before the treatment were apparently higher than that in healthy children of control group, the difference was statically significant.(P <0.01; P <0.01)3. The levels of serum S100 B protein and NSE in children of TD group before the treatment had no significant relationship with the YGTSS.(r =0.222, P >0.05; r =-0.016, P >0.05)4. The levels of serum S100 B protein and NSE in children of TD group after a 12 weeks Aripiprazole treatment were lower than that before the treatment, the difference was statistically significant.(P <0.01; P <0.05)5. Use the YGTSS to evaluate the clinical curative effect of Aripiprazole and the result showed one child was cured, 47 children had significant curative effect, 2 children had curative effect, and 13 children showed no reaction to the treatment. The total effective rate was 80%. Main adverse drug reactions were drowsiness, headache, dizziness. There were no other durg reactions. The incidence of adverse reactions in TD group was 9.52%. Conclusions1. The serum S100 B protein concentration in healthy children and TD patients were not altered as age increases.2. The serum S100 B protein and NSE concentration of TD group children were both higher than that of control group. It illustrated that TD patients existed brain damage.3. The brain damage degree of TD patients had no relationship with tic severity.4. The syptoms of TD patients improved after therapy. The concentration of serum S100 B protein and NSE were lower than that of prior treatment and it illustrated that brain damage of TD patients improved after treatment.5. The symptoms of TD patients improved after using little dose of aripiprazole(2.5mg/d~10mg/d)and the concentration of serum S100 B protein and NSE were lower than before. It illustrated that aripiprazole had certain effect on curing TD patients and could be a new and safe medicine in TD treatment.