Effect Of Curcumin On Angiogenesis Of Hepatocellular Carcinoma Hypoxia Model In Rats

Objective: To investigate the effect of curcumin on angiogenesis in rats after hypoxic hepatocellular carcinoma, we observe HCC vascular endothelial growth factor(Vascular endothelial growth factor, VEGF) and microvessel density(Microvessel density, MVD) expression changes through the establishment of hepatocellular carcinoma hypoxia model in rats and multi-devascularization combined treatment.Methods: We established rats’ primary liver cancer by intermittent feeding diethylnitrosamine(DEN) solution and intraperitoneal injection DEN combined. In order to set up an oxygen-deficient environment, we ligature the hepatic artery of hepatocellular carcinoma model. Then those hepatocellular carcinoma hypoxia model were divided into four groups, group A(10) using of the portal vein catheterization + chemoembolization; group B(10) using only the portal vein catheterization + chemoembolization + curcumin; group C(10) using only the portal vein catheterization + chemoembolization + capsule; D group(10) using the portal vein catheter + capsule + curcumin. After one week of treatment, two rats of each group were sacrificed and the tumors were taken out. Tumor vascular endothelial growth factor(VEGF) and microvessel density(MVD) were detected by immunohistochemical chemical color inside(SP) and Western blotting.Results: Group A of VEGF optical density value, MVD value, VEGF protein expression, the average survival time: 0.84 ± 0.11, 91.5 ± 5.6, 73.1 ± 4.2, 18.33 ± 1.08d;B group of VEGF optical density value, MVD value, VEGF protein expression, the average survival time: 0.69 ± 0.23, 59.3 ± 4.9, 48.9 ± 5.9, 23.44 ± 0.85d;C group of VEGF optical density value, MVD value, VEGF protein expression, the average survival time: 0.79 ± 0.09, 87.1 ± 5.1, 68.4 ± 3.6, 24.57 ± 0.57d;D group of VEGF optical density value, MVD value, VEGF protein expression, the average survival time: 0.64 ± 0.17, 53.9 ± 3.8, 45.7 ± 4.2, 30.63 ± 1.64 d.VEGF optical density and protein comparison: the optical density of A, C group were suppressed and compared with the optical density of B, D group, the difference was significant(P <0.01), the optical density of A was high and compared to the optical density of C group, but the difference was not significant(P> 0.05), the optical density of B was high and compared to the optical density of D group, but the difference was not significant(P> 0.05); the VEGF ptotein of A, C group were high compared to the VEGF ptotein of B, D group, the difference was significant(P <0.01), the VEGF ptotein of A was high and compared to the VEGF ptotein of C group, but the difference was not significant(P> 0.05), the VEGF ptotein of B was high compared to the VEGF ptotein of D group, but the difference was not significant(P> 0.05);MVD value comparison: the MVD value of A, C group were high compared to the MVD value of B, D group, the difference was significant(P <0.01), the MVD value of A was high and compared to the MVD value of C group, but the difference was not significant(P> 0.05), the MVD value of B was high compared to the MVD value of D group, but the difference was not significant(P> 0.05);Animal survival comparison: the median survival time of the intervention group(including B, C, D group) was significantly longer than the simple group(A group)(P <0.05), the median survival time of intervention group(group D) was longer than the single pathway intervention(B, group C)(P <0.05), the median survival time of group C was longer than the group B, but the difference was not significant(P> 0.05).Conclusion:1、Curcumin inhibited VEGF expression by hypoxia and tumor angiogenesis in rats’ primary liver cancer via the portal vein;2、Alone and in combination perihepatitis diaphragm no obvious inhibition of VEGF expression and tumor angiogenesis, but it can be better to extend the lifetime of the rat;3、Curcumin combination with portal vein after liver capsule week effectively reduced VEGF protein expression and inhibit the generation of microvessels, and prolong survival in rats.