The Prevalence Of Astrovirus,Sapovirus And Adenovirus In Young Children With Gastroenteritis In Chongqing

Object Through studying, find out the characteristics of the prevalence of three viruses, known as astrovirus, sapovirus and adenovirus, which can cause gastroenteritis in young children in Chongqing. Studying the difference of the prevalence of the three enteric viruses in categorized age, seasonal, sexual groups can also provide basic data for establishing a strategy to prevent viral diarrhea.Methods Collect fecal samples from 511 children with acute diarrhea (≤2weeks) from January to December in 2014 in CHCMU (Children’s hospital of Chongqing Medical University) outpatient department and record the clinical data of the pediatric patients. Then detect the fecal samples in medical laboratories. RT-PCR,which is short for reverse transcription-polymerase chain reaction,can be used for detection of Sapovirus and Astrovirus’s nucleic acid. PCR is available for examinin adenovirus’s nucleic acid. Analyze the prevalence of the positive samples by categorized month,age, and sex. In addition, among the positive samples,their product after PCR or RT-PCR were purified and sequenced. Calculate the genetic distance by multivariate statistics analysis method and structure a phylogenetic tree.Results 511 specimens were collected from January to December in 2014,. Among these,290 were from males and 221 from females. The positivity to Sapovirus is 4.7%, Astrovirus is 2.7%, and adenovirus is 2.9%. There is no significant difference between females and males for the three viruses. The cold months are the major months of the viral prevalence (astrovirus and sapovirus). Adenovirus can infect children all year round. The results of detection were higher in children between 0-36 months.Conclusion Among the viruses which can cause acute gastroenteritis in children, SLV, HAstrV and HAdV are the common ones in Chongqing. Seasonal analysis showed the major months of the viral prevalence (astrovirus and sapovirus).are cold months. However, adenovirus Adenovirus can infect children all year round. The results of detection were higher in children between 0-36 months. No novel genotypes were found.