The Effect Of Neotype Rectum Mild Hypothermia Cooling Method On Intestinal Bacterial Translocation Of Hypoxic-Ischemic Brian Damage Sprague-Dawley Rats

ObjectiveTo observe the effect of neotype rectum cooling device on bacterial translocation of Hypoxic-ischemic Brain Damage Sprague-Dawley rats, assess security of the method, and provide experimental foundation for clinical application.MethodsThirty healthy adult male Sprague-Dawley rats were randomly divided into Sham-operated group(n=6), normothermia group(OT, n=12) and mild hypothermia group(MH, n=12). Hypoxic-ischemic Brain Damage model were conducted in the C and MH group. The rats of MH group were chosen to connect the rectum cooling device through rectum, cold isotonic saline solution was poured into double-cavity closed water storing capsule constantly to maintain mild hypothermia for 12 hours and then rewarmed in cage. Blood were taken at 0,12,24 hour after ischemia hypoxia to test the concentration of diamine oxidase(DAO) and D-lactate. Rats were sacrificed at 24h after ischemia hypoxia. Spleen, liver, mesenteric lymph nodes were taken from abdomen and cultured on mediums. Rectum was removed and sections were analyzed by HE staining.Results1. Intestinal mucosal morphology:Rectum of C group were complete. The rectal mucosa columnar epithelium of OT group were damaged obviously, some epithelium were missing, plenty of inflammatory infiltrates were found under the mucosa. The rectal mucosa villus of MH group were complete, few inflammatory infiltrates were under mucosa.2.Bacteria culture:The results showed that bacterial translocation was found in all group with no statistical significance (P>0.05). Bacterial translocation was found in Mesenteric Lymph Node (MLN) in C and MT group, only Escherichia coli was cultured. MLN was the major place that BT was found in OT group, BT were found in spleen and liver either, and there was no statistical significance compared with MH group (P>0.05).The places of BT in OT group were the same as the route of BT, it meant route of BT in HIBD rats which did not accepted MH cooling was further. Bacteria translocated in OT group were Escherichia coli, Stenotrophomonas maltophilia, Acinetobacter lwoffii, there was no statistical significance in BT rate in C group compared with MH group (P>0.05)3.Concentration of diamine oxidase(DAO) and D-lactate:The concentration DAO and D-lactate rose at 12h in both OT group and MH group compared with the moment after ischemia hypoxia(P<0.05).Conclusions1.The neotype rectum mild hypothermia cooling method can maintain the integrity of the rectal histology with no rectal injury. Double-cavity closed water storing capsule was adopted, cold water was kept from rectum, and the incidence of water intoxication complications could be avoided.2.The device was demonstrated to maintain intestinal micro ecological balance, with no effect on bacterial translocation of rats suffered from HIBD.3.The method showed good protection of rats suffered from HIBD, which lower intestinal mucosal permeability.4.The results of the former and later animal experiments showed that the neotype rectum mild hypothermia cooling method is safe, portable, inexpensive, simple, noninvasive. Further clinical trials are needed to testify the safety of this method in clinical application.