Effects Of Ligusticum Chuanxiong Hort Drug-Containing Serum On Proliferation And Cannabinoid Receptor 1(CB1) Related Signaling Pathway In Rat Hepatic Stellate Cells

ObjectiveTo investigate the effects of Ligusticum chuangxiong hort drug-containing serum on CB1, FAK, p-Akt and type Ⅰ and Ⅲ collagen expression in rat hepatic stellate cells(HSCs) stimulated by platelet-derived growth factor (PDGF-BB), and explore the mechanism of hepatic fibrosis.MethodsLigusticum chuangxiong hort Hort drug-containing serum was produced, the effect of drug-containing serum on the proliferation of HSCs was detected by MTT assay; the protein levels of CB1, FAK, p-Akt were analyzed by Western Blot; the secretion of collagen Ⅰ and Ⅲ were examined by RT-PCR assay.ResultsCompared to the normal control group, PDGF-BB promoted the proliferation of HSCs significantly, increase the protein levels of CB1,FAK,p-Akt, and improved the level of collagen Ⅰ　and Ⅲ. Ligusticum chuangxiong hort drug-containing serum, colchicine and AM251 could inhibits the above effects in HSCs distinctly. After joining the AM251, the inhibitory effect of Ligusticum chuangxiong hort drug-containing serum was more apparent, and it was in a dose-dependent manner.ConclusionLigusticum chuangxiong hort drug-containing serum can inhibit the proliferation of HSCs and impact the level of collagen Ⅰ and Ⅲ by CB1 related signaling pathway, and thus play the role of prevention and treatment of hepatic fibrosis.