| Objective:To observe the effect of benzene and [a] pyrene exposure 30 days on behavior and toxicity of nerve tissues in male SD rats and the protective effects of vitamin E with different dose on benzo [a] pyrene.To investigate the mechanism of intervention effect of vitamin E(VitE) supplement.The methods used and the results obtained in this paper are important to future researches.Methods:Male SD rats were maintained in the No Specific Pathogen-free (SPF) room in the experimental animal center of Chongqing Medical University. They were randomly assigned to six groups, which consisted of 10 mice per group. As follows:blank control group(double distilled water), solvent control group (vegetable oil),pure treated with B [a] P group (5mg/kg B [a] P), low dosage of VE+B[a]P group(10mg/kg VE+5mg/kg B[a]P), moderate dosage of VE+B[a]P group(50mg/kg VE+5mg/kg B[a]P), high dosage of VE+B[a]P group(100mg/kg VE+5mg/kg B[a]P). The learning and memory abilities were tested by Morris water maze training after 30 days intragastric administration. And then we collected hippocampus the next day. Morphous of the hippocampus were observed after HE stained by microscope. The level of MDA,8-OHdG and the activity of SOD, GSH-Px were tested in the hippocampus.Results:The MWM results showed that the mean escape latency was shortened compared with the blank control group along with the increment of the training days, and also gradually shortened with a rising of the dosage of VE. (P< 0.05); The differences were statistical significance. (P< 0.05).Compared with blank control and solvent control group, the mean escape latency of low dosage VE and B[a]P group have statistical significance(P< 0.05); Compared with B[a]P group, the mean escape latency of VE group has statistical significance. (P< 0.05).The times across platform and time during platform quadrant in control and different doses of VE group were significantly higher than B[a]P group (P< 0.05).HE stained indicated that there were scattered damaging cells in the hippocampus nerve cells, mainly showed that vacuolar degeneration in cytoplasm and around nucleus; The cellular structures were destroyed and the cellular outlines were vaguely. Compared with B[a]P group, there has been a certain amount of improvement in pallium and hippocampus with a rising of VE doses.Compared with bank control group, the levels of MDA were augmented and the activity of SOD, GSH-PX were significantly reduced in the hippocampus of B[a]P group (P<0.05). There was no significant improvement of MDA levels in low and moderate dosage of VE+B[a]P group compared with B[a]P group (P>0.05), while the activity of SOD, GSH-PX were significantly increased (P<0.05), and the level of MDA was significantly reduced and the activity of SOD and GSH-PX was increased(P<0.05).Compared with control group, there was a significant risen of 8-OHdG levels in B[a]P group, but the more dosage of VE supplement,the higher reduced of 8-OHdG levels, Among them,the most lower was the high dosage VE+B[a]P group (P<0.05).Conclusion:There was an obviously Pathologic damage in nerve tissues of male SD rats after B[a]P exposure 30 days and the level of lipid peroxidation damage marker MDA was increased, and the activity of antioxidative enzymes SOD,GSH-Px were reduced, and the level of DNA oxidative damage marker 8-OHdG was risen, and the hippocampus structures and function were destroyed. Lipid peroxidation may be one of a important mechanism, By adding appropriate doses of VE can reverse the toxicity caused by B[a]P, and provide some basis for their prevention and treatment. |
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