| Objective:1.To detect the acaricidal effect of air cleaner;2.Study the effect of air cleaner to reduce the house dust mite allergen concentration and activity;3.Study preliminary the mechanism of air cleaner acaricidal and reducing the allergen activity.Method:1. Air cleaner air purifying dust full kill rate:of adult dust mites at different times (12h,24h,48h,72h) to accept the treatment, each time period of about 200 adult dust mites is observed in the anatomy under a microscopedust mites in the number of deaths and calculate the dust mite mortality;2. The air cleaner to kill dust mites mechanism:will go through 12h,24h,48h, 72h,84h air cleaners deal with death dust mites produce paraffin sections, immunoh-istochemical methods, the positioning of each group dust mites allergens. By HE staining, and scanning electron microscope to observe the morphological changes of circumstances of the death dust mites. Apoptosis experiments, and death, dust mites DNA electrophoresis to detect the apoptosis of each group of dust mites;3. Air cleaner for house dust mite allergens clear role:dust samplers were collected house dust mite allergen in the following four environmental samples:(1) no air conditioning and air cleaners; (2) air conditioning open air cleaner; (3) air conditioners and air cleaners; (4) open air cleaner no air conditioning. Was measured by ELISA in the air, dust mites in four environmental allergens of Der fl,, Der f2, concentration;4. The air cleaner on the dust mite allergen immune activity:the cultivation of dust mites in the medium ground into powder, air purification handles Oh,12h,24h, 48h,72h, detected by ELISA and immunoblotting air cleaner to deal with different time changes of the dust mite allergen immunogenicity.5. Spectral analysis of air purification before and after the processing of spatial structure of β-lactoglobulin:circular dichroism chromatography and fluorescence spectroscopy to measure the air cleaner treated milk β-lactoglobulin circular dichroism and fluorescence spectroscopy, analysis of β-lactoglobulin space changes in the structure of the relationship of the change and changes in the protein immune activity.Result:1. The statistics air cleaner the acaricidal rate results:dust mite mortality extended processing time with the air cleaner and increased after 84h treatment, the mortality rate of 100%. Dust mites in the natural state mortality 84h after only about 10%.2. HE and sweep the surface electron microscopy results show that, dust mites in the body of water of death and serious lost, the whole mite body showing dry flat like, dust mite death is caused by loss of body water; immunohistochemical results showed that the air cleaner to kill dust mites allergens in addition to the intestine in a small amount of the existence of the rest such as parts of the gland, reproductive system, you can not see; TUNEL in situ cell apoptosis detection and dust mite DNA electrophoresis results showed the death of the dust mites in the body cells and apoptosis.3. The 20 sampling room air conditioning, house dust mite allergen content will be increased in the use of air cleaners, whether it is in the air conditioning turned on or off the indoor dust mite allergen Der fl and Der f2 will be lower (P<0.01);ELISA and Western blot results showed that:dust mite allergen in the dust mites in the medium due to the role of plasma generated by the air cleaner, immune activity decreased.4. Circular dichroism and fluorescence spectroscopy have shown that the air cleaner processing of beta-lactoglobulin, its spatial structure, enhanced hydrophobic beta-lactoglobulin, which may be one of the reasons resulting in changes in their immune activity.Conclusion:The air cleaners used in this experiment has to kill dust mites role, and can significantly reduce dust mite allergens in indoor air. At the same time the role of plasma collected in its filters, allergen immunotherapy activity, avoiding secondary pollution. Protein immune activity changes is because plasma caused a change of the protein spatial structure. Dust mites in the air cleaner in the death is due to the severe loss of water, lack of water and died. In this process, dust mite body cells and apoptosis. |
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