Study The Effect Of Estrous Cycle、Sex-hormone And Partial Mechanisms Of Benzoin On Uterine Smooth Muscle Of Non-pregnant And Pregnant Rats And Mice

Objective:This paper aims to study the effect of Benzoinum on estrous cycle、 sex-hormone and to observe the effect and it’s mechanisms of Benzoinum on uterine smooth muscle in health female rats and mouse. To research whether benzoin belongs to pregnancy contraindication or not, and to provide the pharmacological evidence for rational drug use of clinical gynecologic.Methods:①To observe the effect of benzoin on the changes of estrous cycle of mice and rats by vaginal exfoliated cells; ②Estradiol(E2)、progesterone(P) and so on, in serum was measured with enzyme linked immunosorbent assay(ELISA); the index of uterine and ovarian was calculated by weighting mice and rat’s uterus and ovary; ③ The muscle tension frequency and peak areas of early pregnant rats and non-pregnant rats uterine smooth muscle in vivo of Benzoinum in different dosages by us ing bio logical functional system and observation the intervention mechanisms of the uterine smooth muscle of benzoin of 1.0 g·kg-1 on oxytocin (OT) and misoprostol by using BL-420 systemResult:(1) The effect of benzoin on the changes of estrous cycle、sex hormone and the index of uterine and ovarian of mice and rat: ① Benzoin 0.5、1.0 g·kg-1 group were able to make the non-pregnant mice and rats’obviously extend the estrous time, shorten the time of non-estrogen(P<0.05, P<0.01); 0.25、0.5、1.0 g·kg-1 groups not only increased non-pregnant mice serum sex hormone levels of PROG significantly but also increase non-pregnant female mouse the index of ovary (P<0.05, P<0.01); 0.5,1.0 g·kg-1 groups can increased non-pregnant mice and rats’index of uterus serum and improve mice’s sex hormone levels ofPROG significantly (P<0.05, P<0.01); 1.0 g·kg-1 groups can increased non-pregnant mice and rats’serum sex hormone levels of E2 and PROG significantly (P<0.01, P<0.05). ②Benzoin 0.5、1.0 g·kg-1 could increase the early pregnant rat’s serum sex hormone levels of E2 and PROG significantly (P<0.01, P<0.05); 1.0 g·kg-1 could significantly increase the early pregnant rat’s index of ovary and uterus (P<0.05).(2) Effection and mechanism on uterine smooth muscle of non-pregnant rats and early pregnant rats in vivo：① The doses of Benzoin 0.5、1.0 g·kg-1 were chronically administered to rats for 9 days could remarkably decrease the peak area and frequency of non-pregnant rats uterus (P<0.05); 1.0 g·kg-1 also decrease the the tension of non-pregnant rats uterus (P<0.05). ② Benzoin 0.5、1.0 g·kg-1 were chronically administered to rats for 3 days by gavage could remarkably decrease the tension、peak area and frequency of early-pregnant rats uterus (P<0.05, P<0.01); The findings of duodenal administration shows, the doses of Benzoin0.25、0.5、1.0 g·kg-1 could remarkably decrease the frequency of early-pregnant rats uterus (P< 0.05); 0.5、1.0 g·kg-1 could remarkably decrease the peak area of early-pregnant rats uterus (P<0.05); and 1.0 g·kg-1 could remarkably decrease the tension of early-pregnant rats uterus. ③The doses of Benzoin 1.0 g·kg-1 could remarkably obviously inhibit the exciting of the non-pregnant rats and early-pregnant uterus caused by OT (P<0.01), it showed significant difference by given OT again (P< 0.05,P<0.01); it could obviously resist the excitatory contraction of early pregnant uterus caused by misoprostol (P<0.01), it showed no significant difference by given misoprostol again (P<0.01)Conclusion:Benzoin were able to make the non-pregnant mice and rats obviously extend the estrous time, shorten the time of non-estrogen; Benzoin could significantly higher the level of E2 and P in serum and promotion the development of reproductive organs; Benzoin show significantly inhibitory effect on uterus of nonpregnant and early pregnant rats, the mechanism may be related to blocking oxytocin receptor and misoprostol receptor partly.Above all, this research only description that Benzion joined gynecological treating such as dysmenorrheanot, it may be related to regulation of the estrous cycle、 influencing sex-hormones and inhibit the anomaly contraction of uterus smooth muscle. Partly reflect of the benzoin at rats’ uterus and hormone levels of early-pregnancy without ill effects, but wheather it has bad effection on pregnancy fetal, during late pregnancy or not, it still need research in-depth and systematic studay on the safety and efficacy of benzoin in Gynecology in the future.