Influence Of Astragalus Polysaccharide On Apoptosis Of Cervical Carcinoma C-4I Cells

Background and ObjectivesLike other tumors, apoptosis plays an important role in the occurrence and development of cervical carcinoma. So, it is important to explore the mechanism and the ways to induce apoptosis of cervical carcinoma cells for treatment of cervical carcinoma. Asragulus polysacharin (APS) is the main active ingredient of astragalus (a commonly used traditional Chinese medicine). It has been confirmed that APS could inhibit the growth and invasion of diverse tumor cells. But, the influence of APS on apoptosis of cervical carcinoma C-4I cells have not been reported yet. This study intends to investigate the influence of APS on the apoptosis of cervical cancer C-4I cells and its mechanism, and provide the experimental evidence for the clinical application of APS in the treatment of cervical carcinoma.Methods1. APS was prepared, then its concentration is detected.2. Based on the results of the pre-experiment, in this study, cervical carcinoma C-4I cells treated with 200μg/mL APS for 48h as the experimental group, cervical carcinoma C-4I cells untreated with APS as the control group.3. The apoptosis of cervical carcinoma C-4I cells before and after treated with APS was detected by Annexin V-FITC/PI combined with flow cytometry and Terminal-deoxynucleotidyl transferase mediated nick end labeling (TUNEL).4. The expressions of Bcl-2, Bcl-XL, Bax, Smac and IAP-1 genes in cervical carcinoma C-4I cells treated and untreated with APS were detected by Western blotting assay.Results1. The quantitative result of prepared APS solution was 61.56mg/mL. According to the results of effects of Astragalus Polysaccharide on the Growth of Cervical Carcinoma C-4I Cells, in this study,the experimental group was set to cervical carcinoma C-4I cells treated with 200μg/mL APS for 48h, while the control group was set to cervical carcinoma C-4I cells untreated with APS.2. AnnexinV-FITC/PI combined with flow cytometry results suegested that the apoptosis cells of cervical carcinoma C-4I cells treated with 200μg/mL APS for 48h in experimental group were significantly increased than that cervical carcinoma C-4I cells untreated with APS in control group (P<0.01). The apoptotic cells percentages of cervical carcinoma C-4I cells in control group and experimental group were 24.138% and 0.583%, respectively.3. The results of TUNEL indicated that the apoptosis cells of cervical carcinoma C-4I cells treated with 200μg/mL APS for 48h in experimental group were obviously increased than that cervical carcinoma C-4I cells untreated with APS in control group (P<0.01).4. Western blotting results showed that:The expression of Bcl-XL was no significant difference in cervical carcinoma C-4I cells treated with 200μg/mL APS for 48h in experimental group and that cervical carcinoma C-4I cells untreated with APS in control group (P>0.05). The expressions of Bax and Smac in cervical carcinoma C-4I cells of the experimental group were significantly higher than that in cervical carcinoma C-4I cells of the control group(P<0.01). The expressions of Bcl-2 and IAP-1 in cervical carcinoma C-4I cells of the experimental group were significantly lower than that in cervical carcinoma C-4I cells of the control group(P<0.01).ConclusionsAPS can promote the apoptosis of cervical carcinoma C-4I cells by down regulating the expressions of Bcl-2 and IAP-1 and up regulating the expressions of Bax and Smac.