Peroxiredoxin I Protects Pancreatic β Cells Apoptosis From STZ

Type I diabetes is mainly caused by oxidative stress, immune dysfunction, genetic factors, microbiological and viral infections as well as other factors which will lead to damage of insulin-producing pancreatic β cells, and abnormally insulin secrete, resulting in hyper blood glucose metabolic disorders; Prx I is a kind of superoxide peroxide which widely exists in cytoplasm, it has important roles of clearing relative lower level of reactive oxygen within cells. By examining the protective role of Prx I scavenging intracellular reactive oxygen species on the pancreatic β cell protection, confirm the mechanism of pancreatic β cell apoptosis in the situation of oxidative stress caused by Prx I gene deletion, has important significance to clear the pathogenesis of type I diabetes.This experiment by study on diabetes model animals which is Prx I gene knockout mice injected with STZ and induced type I diabetes found that: Prx I has a significant protective effect on mouse pancreatic β cells death induced by STZ injection; by intraperitoneal single high dose injecting STZ, 48 hour wild type and Prx I gene knockout mice have reached a minimum target of diabetes in blood glucose level, 96 hour Prx I gene knockout mice all died, pathological histology testing showed that a lot of Prx I knockout mouse pancreatic β cells necrosis, some of the pancreas center renders a blank area; the results in vitro showed that: STZ treated Prx I gene knock and normal pancreatic island beta cells did not appear obviously changes in cell cycle replicative phase and mitosis phase, but Prx I gene knock down cell line appears obviously increase in the radio of Sub G0 period cell, by using apoptosis detect kit confirmed that Prx I knock-down pancreatic β cell undergoing a obviously apoptosis, Western blotting detection reactive oxygen related signals and apoptosis related signals found that antioxidant protein SOD, apoptosis relative protein Caspase3 express highly increased, so in conclusion Prx I is by adjusting the level of cellular reactive oxygen inhibition of pancreatic β cell apoptosis; the results in virto showed that Prx I can become a diabetes prevention and treatment potential targets.