| Mammal life cycle is longer, which often leads to gene knockout totally embryonic lethality is not conducive to the cardiovascular system research and observation. Zebrafish small size, can provide oxygen and nutrients by osmosis, even if there are serious cardiovascular defects can survive for some time. Its embryonic transparent, easy to detect and observe the process of cardiac development in zebrafish and humans are highly evolutionarily conserved, it is one of the ideal model scholars recognized research and development of biological heart and cardiovascular. Our previous discovery HFHG45 regulation of blood and blood vessel development. In this paper, the model organism zebrafish gene HFHG45 further study the impact on the hematopoietic system development. According to reports, the number of incidence of cardiovascular and cerebrovascular diseases ranks first major country, but most of our mortality diseases. The occurrence and development of many human diseases are associated with the development of blood vessels or have a very close and inextricably linked.Vertebrates and blood vessels are derived from the same cell that progenitor cells of the blood. Blood progenitor cells originated in the mesoderm, and then differentiate into vascular progenitor cells and blood progenitor cells. By the blood progenitor cells from the blood cells mainly involved in the early pre-hematopoietic progenitor cells derived from the blood of the regulation of hematopoietic stem cells, differentiate into mature myeloid cells, red blood cells and lymphocytes. Vascular progenitor cells begin to differentiate into vascular endothelial(EC) cell, after differentiation of arteries, veins and lymph vessels and the like. Blood vessels and the development process is under the control of complex and orderly conduct of the molecular signaling networks, which can cause serious abnormal developmental defects or serious illnesses. Currently, the molecular mechanism of regulation of the hematopoietic system and the vascular system of research and development is far from clear, and to explore the blood vessels during development of important molecules help improve blood vessels during development and molecular signaling network, for the treatment of heart and cardiovascular disease to provide means and methods of the new personalized treatment.In this paper, to explore the impact HFHG45 for vascular development and differentiation of hematopoietic stem cells by zebrafish model. Our previous established stable HFHG45 knockout strains. In this paper, the use of molecular probes to detect signs of different types of blood cells, hematopoietic stem cells and blood vessels in the marker gene knockout strains HFHG45 complete expression. Mainly related marks Notch signaling, shh signal and other important signaling pathway genes were detected. The main detectable marker gene as follows: shh and myod1(somite cell marker gene), dld and dlc(notch signal ligand), pax7a(Jisheng ganglion cell marker genes), pax2 a and cdh17(prerenal marker gene), amhc( cardiac marker gene), scl and etv2(hematopoietic stem cell marker gene). The results showed that the expression knock shh, scl, dld, dlc, pax7 a, pax2 a, cdh17, amhc, etv2 and myod1 addition HFHG45 significantly lowered in zebrafish.In summary, HFHG45 possible by Notch signaling and shh signaling regulates cell differentiation and blood vessel development, but its effect on the heart, including atrial and ventricular impact is not obvious. |
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