Effect Of FOXQ1 Gene Silencing On EMT Of Colorectal Cancer SW480 Cells And Its Mechanism

Objective:To explore the effect of FOXQ1 gene on EMT of Co lorectal Cancer SW480 cells and its mechanism.Methods:Short-hairpin RNA (shRNA) of FOXQ1 and Negative Control shRNA were constructed and transfected into SW480 cells w hich express high level of FOXQ1.After the lentivirus with the best s ilencing effect was screened,it would be the only silencing one in fol lowing experiments.Next,the SW480 cells were set as Interfered grou p、Negative group、Control group.Cellular morphology and the junction among cells were examined under the microscope,transwell chambers assay、adhesion experiments were used to detect the ability of invasi on、migration、homogeneous and heterogeneous cells intercellular adh esion of each group.Meanwhile,qRT-PCR、Western blot were used for analyzing E-cadherin、N-cadherin、Vimentin and MMP-2 expression.Results:The lentivirus with FOXQ1-sh1 successfully silenced th e FOXQ1 gene of SW480 cells.Compared with Negative group and Control group,Interfered group extensive cells polarity and tight juncti on among cells increased;invasion and migration ability were weaken (P<0.05);homogeneous cells intercellular adhesion ability was increa sed and heterogeneous cells intercellular ability was opposite(P<0.05); meanwhile,the expression of E-cadherin declined significantly (P＜0.05), the expression of N-cadherin、Vimentin and MMP-2 rose significantly (P＜0.05).Conclusion:Silencing FOXQ1 gene can reverse the EMT of the colorectal cancer SW480 cells and inhibit cell metastasis,its mechani sm may be related to the up-regulation of E-cadherin and down-regul ation of N-cadherin、Vimentin and MMP-2.