Developmental Potential Of The In Vitro Matured Oocytes From Controlled Ovarian Stimulation Cycles And Mitochondrial Membrane Potential Detection Before Insemination

Sectionl The effect of gonadotrophin on the in vitro maturation of immature oocytes from controlled ovarian stimulationObjective To evaluate the effect of FSH/HCG on in vitro maturation of immature oocytes obtained from controlled ovarian stimulation (COH) cycles.Methods MI stage oocytes and GV stage oocytes were collected from COH cycles for intracytoplasmic sperm injection (ICSI) treatment in this study. ALL of these immature oocytes were divided into two groups and cultured for 24 to 28 hours in insemination media. FSH/HCG culture group contained 0.075IU/mL FSH and 0.15IU/mL HCG. Matured oocytes were given ICSI. The rates of maturation, normal fertilization, cleavage, and high-quality embryo were recorded and compared.Result For MI oocytes, there were no difference between the two groups in maturation rate, fertilization rate, cleavage rate and high-quality embryo rate (P>0.05). For GV oocytes, high-quality embryo rate in FSH/HCG culture group was higher than that of no FSH/HCG culture group (31.3%VS4.2%, p<0.05).Conclusion Immature oocytes from COH could mature in vitro whether adding hormones or not. During the maturation of the GV stage oocytes, FSH/HCG could increase the developmental potential of the matured oocytes.Section2 Effects of mitochondrial membrane potential detection on development potentiality of human oocytes matured in vitroObjective By laser confocal microscopy observation obtained at different time, different incubation time in vitro maturation of oocytes of mitochondrial membrane potential, assessment of mitochondrial membrane potential detection of in vitro maturation of oocyte developmental potential application value.Methods MI stage oocytes and GV stage oocytes were collected from COH cycles for intracytoplasmic sperm injection (ICSI) treatment in this study,randomly divided into two groups, respectively in 24 hours and 48 hours later with a laser confocal fluorescence microscopy after mature by JC-1 (5,5’,6,6’-tetrachloro-1, 1’,3,3’-tetraethylbenzimidazolcarbocyanine iodide) dyeing phase of MII oocytes of mitochondrial membrane potential, the red fluorescence distributed three states: circumferential pericortical; punctuate distribution within the cytoplasm, scant.Result 24 hours of mature oocytes and 48 hours in mature oocytes, although with the extension of time, the distribution of the surrounding Billy is greatly reduced, but compared with other distribution results no significant difference (P> 0.05).For GV stage oocytes, D1 and D2 days mature oocytes of mitochondrial membrane potential of fluorescence distribution without significant difference (P> 0.05).For MI oocytes, relative to the D1 day, D2 day mature oocytes, mitochondrial membrane potential distribution around less, but compared with the mature oocytes D1 day without significant difference (P>0.05).Conclusion Mitochondrial membrane potential detection can only part of the reaction activity of in vitro maturation of oocytes and development potential, we observed that delay earlier mature oocytes mature oocytes of mitochondrial membrane potential is lower and a reduction in the ability to reflect the metabolism of oocytes, can to a certain extent, indicates its development potential.