| Vibrio parahaemolyticus (VP), Gram-negative, halophilic brevibacterium, coastal waters, as well as fish and shellfish Hehai junction seafood common strains of Vibrio parahaemolyticus has become the cause of the main bacteria-food borne diseases, only after the Vibrio cholerae harm to humans. In recent years, food poisoning infection caused by Vibrio parahaemolyticus has become a very serious public health problem worldwide. The World Health Organization has reported that since 1997 Vibrio parahaemolyticus caused various diseases shows a continued rise trend, especially in China and Japan. Vibrio parahaemolyticus infection in animals often cause sporadic and endemic gastroenteritis, sometimes causing wound infections and sepsis and a series of severe clinical symptoms, but Vibrio parahaemolyticus pathogenesis is not yet very clear that there is some controversy. Therefore, the present study mainly through Jiangsu Province 63 strains in the library (Vibrio parahaemolyticus, VP) performed serotyping, multilocus sequence typing and virulence factor analysis carried,to understand the epidemiology of Vibrio parahaemolyticus.1. The survey for the serotype distribution of Vibrio parahaemolyticusVibrio parahaemolyticus antigenic structure is extremely complex,and the analysis of environmental pathogenic strains and serotype distribution and interrelation of clinical isolates of Vibrio parahaemolyticus, with a series of very important significance for prevention and treatment of diseases caused by VP. According to epidemiological investigation,we have found that serotype distribution of Vibrio parahaemolyticus in recent years has undergone major changes, with the external environment to identify serotype distribution and internal relations of VP populations, has important implications for disease prevention.The pop dynamics in 1997 winter -related causes by epidemic clone of Vibrio parahaemolyticus in Peru,03:K6 serotype first occurred in 1996 in India Kalka A, this new serotype is considered to be the end of the 1990s, a global pandemic important serotypes, then these strains spread rapidly to other countries. The serum used in the experiment slide agglutination test for collected 61 environmental isolates of Vibrio parahaemolyticus strains from 2006 to 2012 of serotype distribution of a detailed investigation, monitoring points covering Yangzhou, Taicang, Changzhou, Changshu, Huaian, Kunshan, Jiangdu, such as the East, Sihong, Taizhou, Wuxi and other 11 regions.The isolates is mainly from the deli and seafood, experimental identification serum of Vibrio parahaemolyticus were purchased from Japan Health Research Corporation.The survey found that five new serum type combination, namely 01:K33,01:K68,02:K25, O11:K31, including that two strains of O antigen is not finalized,24 K antigens are not stereotypes, typing rate was 98.36%, mainly contains eight serotypes in 01,02,03,04, 05,08,010 and O11,01 type which accounted for 31.14%,02 type accounted for 18.03%,05 type accounted for 16.39%, and no dominant bacteria type.2. Multilocus sequence typing for the study of Vibrio parahaemolyticusThe experiment by multilocus sequence typing of Vibrio parahaemolyticus isolates typing tests, found 1 dnaE alleles,4 gyrB alleles,5 recA alleles,2 pntA alleles,4 pyrC alleles, a total of 22 new ST type, which has five strains because some alleles do not recognize and can not be completed genotyping. Multilocus sequence typing has better resolution and is widely used in the analysis of typing of pathogenic microorganisms, great significance in terms of traceability and the study of biological evolution.3.Distribution of virulence genes of Vibrio parahaemolyticus analysisThe test by designing primers for PCR gene amplification found that all the environmental isolates do not carry both tdh and trh genes,while the detection rate of toxRS/new geneis is 24%, which plays biological significance in mechanism and further pathogenic of Vibrio parahaemolyticus. |
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