| Study objectives The relationship between gr/gr deletion and spermatogenesis dysfunction was analyzed in order to provide clinical theoretical basis.Study methods 1. The objects in this study were the spermatogenesis dysfunction patients who were diagnosed in First Affiliated Hospital of Jilin University Reproductive Centre from 2012 to2015 except for who karyotypes abnormal, conventional AZF gene microdeletion and other clear pathogenesis(including genital system infection, endocrine system disease, immune system disease, varicocele and obstructive azoospermia). There were 97 spermatogenesis dysfunction patients in experimental group(68 azoospermia patients and 29 oligospermia patients) and 79 sperm donors whose semen were normal in control group. Through multiple PCR amplification, we tested the status of gr/gr deletion and analyzed the relationship of their serum reproduction hormone, seminal plasma biochemistry, testis volume and male infertility.2. Case-control studies about gr/gr deletion and Chinese population spermatogenesis dysfunction in the databases of Pub Med, CNKI, VIP science technology periodicals and WANFUN digital platform etc.(2007, Jan.to 2015, Dec.) were retrieved, furthermore,software Rev Man4.2 was used to statistical analysis.Study results 1. Among 97 spermatogenesis dysfunction patients in this study, we totally examined out 10 patients with gr/gr deletion. The incidence was 10.42%. There were 7 patients with gr/gr deficiency in 68 azoospermia patients. The incidence was 10.27%. We also examined out 3 patients with gr/gr deletion in 29 oligospermia patients. The incidence was 10.34%. In the normal control group, 3 patients with gr/gr deletion were examined out in 79 spermdonations. The incidence was 3.80%. The dyszoospermia group, azoospermia group and oligospermia group compared to control group respectively, the P values were 0.086, 0.109 and 0.194.2. There are 7 gr/gr deletion and 61 gr/gr un-deficiency patients in 68 azoospermia patients. Comparing these two groups ages, FSH, LH, PRL, E2, T, INHB, Seminal plasma fructose, alpha-glycosidase, Seminal plasma zinc etc., the P values were 0.843, 0.194, 0.533,0.913, 0.796, 0.859, 0.788, 0.568, 0.441, 0.406. All of them had no statistic difference.Among 29 oligospermia patients, there were 3 gr/gr deficiency and 26 gr/gr un-deficiency.Comparing these two groups ages, FSH, LH, PRL, E2, T, INHB, Seminal plasma fructose,alpha-glycosidase, Seminal plasma zinc etc., the P values were 0.208, 0.451, 0.617, 0.477,0.990, 0.926, 0.947, 0.654, 0.657, 0.883. All of them had no statistic difference.3. Meta analyzing results: among the study data about spermatogenesis dysfunction which included these 8 results of this research. Heterogeneity test showed no heterogeneity:(χ2=5.66,P=0.58), applying fixed effects model, OR combination = 1.19,( 0.92,1.54), Z=1.30, P=0.19. Result was regarded as gr/gr deletion had no close relationship with spermatogenesis dysfunction.4. Meta analyzing results: among the study data about azoospermia which included these 7 results of this research, heterogeneity test showed no heterogeneity:(χ2=1.81,P=0.94),applying fixed effects model, OR combination=1.10,(0.88, 1.48), Z =0.62, P =0.54.Analysis result was regarded that it couldn’t confirm gr/gr deletion was a dangerous factor to azoospermia.5. Meta analyzing results: among the study data about oligospermia which included these 6 results of this research, heterogeneity test showed no heterogeneity:(χ2=0.90,P=0.97), applying fixed effects model, OR combination= 1.01,(0.71, 1.44), Z =0.06, P=0.95.Analysis result was deemed that it couldn’t prove gr/gr deletion was a dangerous factor to oligospermia.Study conclusion The research revealed that gr/gr deletion was not regarded as a dangerous factor forspermatogenesis dysfunction(azoospermia, oligospermia). Through analyzing parameters of serum reproduction hormone level, seminal plasma biochemistry and INHB level etc., the study showed that gr/gr deficiency couldn’t cause every index obvious variation. |
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