Association Between PLA2r, HLA-QDA1 Gene Single Nucleotide Polymorphism And Genetic Susceptibility To Idiopathic Membranous Nephropathy In Uygur

Objective: To investigate the Correlation between M-type phospholipase A2 receptor(PLA2R) rs 35771982, human leukocyte antigen(HLA-DQA1) rs2187668 single nucleotide polymorphism and idiopathic Uighur membranous nephropathy(IMN).Method: A total of 135 Xinjiang Uygur were enrolled,which included 45 patients with IMN diagnosed by the kidneys pathology and clinical data, 45 patients with IgA nephropathy and 45 healthy controls.The polymorphism of rs35771982 in PLA2 R and rs2187668 in HLA-DQA1 gene were determined with direct sequencing.Using ELISA detect serum anti-PLA2 R antibody in patients with IMN.Result: Rs35771982 locus C gene allele frequency in IMN group was significantly higher than that in the healthy control group(2? = 28.23, P <0.001), in the dominant model, the risk to IMN of whom carrying CC genotype of rs35771982 is 7 times than the CG + GG genotype(OR = 7.00,95% CI = 2.74-17.87).in the recessive model, the risk to IMN of whom carrying GG genotype of rs35771982 is 0.13 times than the CC + CG genotype(OR = 0.13, 95% CI =0.04-0.44).Rs35771982 locus C gene allele frequency in IMN group was significantly higher than IgAN group(2? = 12.10, P = 0.001).in the dominant model,the risk to IMN of whom carrying CC genotype of rs35771982 is 3.63 times than the CG + GG genotype(OR = 3.63, 95% CI = 1.52-8.65).in the recessive model, the risk to IMN of whom carrying GG genotype of rs35771982 is 0.27 times than the CC + CG genotype(OR =0.27, 95% CI = 0.08-0.91).PLA2 R gene loci rs35771982 CC genotype compared with CG+GG genotype in IMN group, 24 h proteinuria was significantly higher( P <0.05),serum albumin significantly lower( P <0.05), the difference of positive rate in anti-PLA2 R antibody was not statistically significant( P> 0.05).Conclusion:The CCgenotype and C allele at rs35771982 in PLA2 R gene are both xinjiang uygur IMN susceptibility genes and functional genes,and CC genotype are positive correlation with proteinuria,negatively correlated with serum albumin and no relevant to positive rate of anti-PLA2 R antibody.