Comparative Analysis Of The Effects Of TNF-α On Proliferation Of SCAP And PDLSCs In Vitro

Objective:The rat cells from the apical papilla and periodontal ligament stem cells were cultured in vitro. The aim of this study was to investigate the effects of tumor necrosis factor-α on proliferation of these cells and explore the possibility t-o continuing development of the root of young permanent tooth with apical period-ontitis. METHODS:The apical papilla, as well as the periodontal ligament tissues from the mandibular tooth of young rats were digested in Hanks solution including collagenase and dispase. Characterization of the immunophenotype of stem cells fr-om apical papilla and periodontal ligament stem cells was detected by immunofluo-rescence technique. Compared the growth curves of their second passage by MTT and drew their growth curves. The third passage cells were challenged with different concentration of tumor necrosis factor-α and detected for the proliferation activit-y by MTT. RESULTS: Stem cells from apical papilla and periodontal ligament stem cells all expressed fibroblastic morphology and the characteristic molecules of s-tem cells. The absorbance value of these cells increased with the increasing of time. Since the forth day, the stem cells from apical papilla proliferation capacity was significantly stronger than periodontal ligament stem cells. At the concentration of 10ng/mL and 50ng/mL, tumor necrosis factor-α can enhanced the proliferating activity of stem cells from apical papilla and also can enhanced the proliferating act-ivity of periodontal ligament stem cells. Stronger proliferation activity of stem cell-s from apical papilla at the concentration of 10ng/mL and 50ng/mL. CONCLUSIONS: Stronger proliferation activity of stem cells from apical papilla, especially un-der inflammation. The results provided experimental basis to these cells which pos-sibly be benefit to continuing development of the root of young permanent tooth with apical periodontitis.