Study On The Extraction And Purification Technology And Quality Evaluation Of Xinjiang Stevia Rebaudiana Bertoni

Objective: To study the purification process through the extraction evaluation of Xinjiang Stevia rebaudiana Bertoni.medicinal quality standard and stevia effective components, the establishment of the Xinjiang Stevia rebaudiana Bertoni. quality evaluation method and quality control, establish the research basis for the rational development and utilization of Xinjiang Stevia rebaudiana Bertoni.Methods: According to the microscopic identification of the system of the original plant of Xinjiang Stevia rebaudiana Bertoni. Chinese Pharmacopoeia 2015 edition, and the description of Stevia rebaudiana Bertoni.; Medicinal water extract, ethanol extract and petroleum ether extract were systematically pre experiment; The same batch of three Xinjiang Stevia medicinal water, the determination of total ash, acid insoluble ash, water soluble extract and alcohol soluble extract; Total polyphenol by UV Stevia rebaudiana Bertoni. was determined by visible spectrophotometry, the content of polysaccharide was investigated; Stevia rebaudiana Bertoni. with the single factor experiments and orthogonal test method Optimization of extraction process of effective components in chrysanthemum, and the use of high performance liquid chromatography of the effective components were determined; Using high-speed countercurrent chromatography were separated in two.Results: The effective components may contain amino acids, Xinjiang Stevia rebaudiana Bertoni. extract polypeptide, protein, sugar, polysaccharide, glycoside, organic acids,flavonoids, phenols, coumarin, sterols, terpenoids and volatile oil constituents; Stevia rebaudiana Bertoni. medicinal water does not exceed 9% of the maximum, the maximum not more than 10% of total ash, acid insoluble ash, a maximum of more than 10%; The water soluble extract and alcohol soluble extract content can reach Pharmacopoeia Ye Juzhong requirements; Stevia rebaudiana Bertoni. content(mainly RA) as index, effects of process optimization of Stevia glycosides extraction time was 1 h, 2 times of extraction,solvent was 20 times; By using high speed countercurrent chromatography combined with high performance liquid Phase separation and identification of the contents of two main glycosides in Stevia rebaudiana Bertoni.Conclusion: This study preliminary explorationof the methods for evaluating the quality of Xinjiang Stevia rebaudiana Bertoni.,established the content of Stevia stevioside and Lai Bao tothe a determination and analysis method, effects of Stevia were the main component of the optimized extraction process,the two effective components of Stevia by high-speed countercurrent chromatography separation, provides a certain scientific basis for quality evaluation of Xinjiang Stevia rebaudiana Bertoni.