CD44v6 And Vimentin Are Associated With Chemo-sensitivity In Paclitaxel Or Doxorubicin Treated Breast Cancer Cells

Background:Breast cancer is an intrinsically heterogeneous in women malignant tumor. About 1 million cases of breast cancer are diagnosed annually in the world. The triple negative breast cancer(TNBC) is a heterogeneous type with a high mitotic rate, they comprise 15%-20% of all breast cancers. Characteristic feature of TNBC is that it lacks expression of oestrogen, progesterone and human epidermal growth factor receptor-2/neu receptors. Chemoresistance after chemotherapy leads to local progression and distant metastasis, which contributes to the failure treatment of patients with TNBC. Objectives:This research is to compare with the different expression of CD44v6 and Vimentin after breast cancer cells treated by PTX and ADM respectively. To explore the changes of the sensitivity to chemotherapeutic drugs of breast cancer cells treated by chemotherapy. To analysis the causes and possible mechanisms of chemoresistance, and to find a valuable molecular therapeutic targets. Methods:CCK-8 kit was applied to detect breast cancer cells’ inhibition susceptibility to different concentrations of PTX or ADM after and before chemotherapy. Western blotting were utilized to evaluate expression of CD44v6, Vimentin after treatment by PTX or ADM. Tumorigenesis potential were measured by a sphere formation assay, and the difference of spheres morphology was observed by inverted microscope. All data were statistically processed by the GraphPad Prism 6.0 Software. Results:Treatment with different concentrations of PTX or ADM resulted in cell growth inhibition of different degrees, in a dose-dependent manner, and breast cancer cells showed different reactions to different drugs. Expression of CD44v6 and Vimentin were increased in chemotherapy treated cells. When compared to the PTX group or ADM group, control group had the relatively small number of cells capable of forming spheres in the population, while there was no significant difference between PTX group and ADM group. Morphologically, the spheres formed in control group tended to be smaller and looser than those in the PTX group and ADM group. Conclusions:Our findings demonstrate that high expression of CD44v6 and Vimentin, along with the enhancement of cells stemness, were associated with chemoresistance in TNBC cell line. CD44v6 and Vimentin may participate in the regulations of many EMT and CSCs pathways, suggesting that they are novel therapeutic target for TNBC treatment.