| Objective:For the purpose of investigating the expression of interferon lambda (IFN-λs) in the serum and analyzing the correlation between IFN-λ, expression and HBsAg, HBeAg concentrations in patients with Chronic Hepatitis B (CHB), We detect the expression of Interferon-λs(IFN-λ,including IFN-λ1, IFN-λ2 and IFN-λ3) in the serum of patients with Chronic Hepatitis B (CHB) and conduct specific fractional analysis according to the concentrations of HBV DNA and Hepatitis B virus e antigen (HBeAg).Thus offering theoretical basis for the in-depth knowledge of the specialty of expression and regulation of IFN-λs in patients with Chronic Hepatitis B.Methods:1. Clinical specimen collection:A total of eighty patients with chronic HBV infection were recruited in experiment, who were in hospital of Shandong Provincial Qianfoshan Hospital during October 2014 to October 2015, of which conduct specific grouping according to the concentration of HBV DNA and HBeAg. HBV DNA level was assayed under fluorescence quantitative PCR, with 40 cases of HBV DNA positive samples. The other 40 cases whose HBV DNA were less than the detected limitation (<100 IU/ml) were divided into negative group. Among which including 20 cases of HBeAg positive and 20 cases of HBeAg negative group respectively. In addition go ahead fractional analysis on the basis of the concentration gradient of HBV DNA. Twenty cases of healthy volunteers were recruited as control. The levels of IFN-λS (including IFN-λ1, IFN-λ2, IFN-λ3), HBeAg and HBsAg were detected respectively by Enzyme-linked immunosorbent assay (ELISA), and correlations between IFN-X, and HBsAg, HBeAg were also analyzed.2. HBV DNA detection:HBV DNA level was assayed under fluorescence quantitative PCR, the lower limit of detection is 100 IU/ml, amplification in strict accordance with process setting of ABI Prism 7300.3. Serologic markers of HBV:HBsAg, HBeAg, anti-HBs, anti-HBe and anti-HBc were all detected with full automatic electrochemiluminescence immunoassay systems of cobas e601.4. Concentration determination of IFN-λs:The concentration of IFN-λ1, IFN-λ2, IFN-X3 were detected by Enzyme-linked immunosorbent assay (ELISA), and the ELISA Kit purched from Shanghai Yuanye Co., Ltd..The concrete operations were in strict accordance with the instruction.5. Statistical analysis:The data were analyzed by SPSS 17.0 statistical software, of which measurement data was show by mean±standard deviation(x±s).Differences between the mean values of two subgroups were evaluated using Independent-Sample T Test.A level of.P<0.05 was considered statistically significant.Results:1. The expressions of IFN-λs in HBV infected samples were all significantly increased compared with that in control group (P<0.05).2. In HBV DNA negative group, the IFN-λ contents of HBeAg positive samples were significantly higher than that in HBeAg negative samples (P<0.05).3. In HBV DNA positive group, the IFN-λ contents of HBeAg positive samples and the HBeAg negative samples were no difference statistically significant(P>0.05).4. In HBeAg positive group, IFN-X levels of HBV DNA positive samples were significantly lower than that in HBV DNA negative samples (P<0.01), especially in HBV DNA higher than 108 samples whose IFN-λs reduced significantly (P<0.05).Conclusion:1. Our research confirmed the expressions of IFN-λs in HBV infected samples were all significantly increased compared with that in control group, which implied chronic HBV infection may stimulate the body’s own expression of IFN-Xs, resistance the infection of chronic HBV.2. When the HBV DNA is negative, the IFN-Xs contents of HBeAg positive samples were significantly higher than that in HBeAg negative samples, prompting when HBV DNA was below detection limit, HBeAg positive group still have low replication of HBV in the body, and can stimulate the expression of IFN-λs.3. The experiment reveal in HBV DNA positive group, the IFN-λs contents of HBeAg positive samples and the HBeAg negative samples were no statistically different. In HBeAg positive group, the higher of the concentration of HBV DNA, the lower expression of IFN-λs. The low duplication of HBV may stimulate the expressions of IFN-λ, while inhibition of IFN-λ expressions may occurred when HBV DNA level high to a certain extent. Moreover the expression of IFN-λs closely related to the body’s immune state. |
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