The Effects Of Methylation Inhibitor 5-AZA-CDR On DNMT1, C-MYC, MKI-67 And P53 Gene Expression In Human Lung Cancer Cells A549

Objective To investigate the effects of methylation inhibitor 5-aza-2 ’deoxycytidine (5-Aza-CdR) on expression of DNA methyltransferase-1 (DNMT1), c-myc, MKi-67 and P53 gene in non-small cell lung cancer A549 cells. So as to reveal the relationship between the gene hypomethylation or demethylation and the development of the mechanism of lung cancer.Methods Lung cancer cell line A549 cells were treated with 5-Aza-CdR as the experimental group,without drugs as the control group,after 5-Aza-CdR treatment for 72 hours,the expression of DNMT1, c-myc, MKi-67 and P53 mRNA were detected by FQ-PCR, the expression of DNMT1, c-myc, MKi-67 and P53 protein were detected by Western blot.Results 1、Methylation inhibitor 5-Aza-CdR treated lung cancer A549 cell lines, as compared with the control group, the experimental group of lung cancer A549 cells was significantly inhibited.2, By FQ-PCR,DNMT1、c-myc和MKi-67 mRNA expression level were 0.35±0.02、0.33±0.03、0.29±0.01 in the experimental group,the gene mRNA expression level were 0.35±0.02、0.33±0.03、0.29±0.01 in the control group;and P53 mRNA expression leve were 1.59±0.04 in the experimental group,P53 mRNA expression leve were 0.89±0.09 in the control group (P<0.01)3、By Western blot,DNMT1、c-myc and MKi-67 protein expression level were 0.43±0.03、0.45±0.05、0.33±0.03 in the experimental group,the protein expression level were 1.21±0.06、1.31±0.12、0.51±0.09 in the control group,and P53 protein expression leve were 1.92±0.05 in the experimental group,P53 protein expression leve were 0.70±0.02 in the control group (P<0.01)Conclusion 1、5-Aza-CdR can inhibit the growth of the lung cancer cell A549.2、5-Aza-CdR can result in the reduce of DNMT1、c-myc、MKi-67 expression,and result in the increase of P53 expression in the lung cancer cell line A549 cells,which is related to genes promoter demethylation.could as theoretical basis for treatment of lung cancer.3、The lung cancer cell hypomethylation or demethylation can inhibit the expression of oncogenes, promote the expression of tumor suppressor gene, thus inhibiting the growth, reproduction and development of lung cancer tumor cells.