The Protective Mechanism Of Anti-macrophage Migration Inhibitory Factor Monoclonal Antibody On Mice With Ulcerative Colitis

Objective:To investigate the protective effect and possible mechanism of anti-macrophage migration inhibitory factor monoclonal antibody(anti-MIF Ab) on mice with acute ulcerative colitis (UC) induced by dextran sulfate sodium (DSS). Methods:Forty BALB/c mice were randomly allocated into normal group, UC model group, anti-MIF Ab group and saline group. All the mice were feed with 5% dextran sulfate sodium solution for seven days except normal group. For the modeling, mice were given intraperitoneal injection of anti-MIF Ab and saline with the same volume at 1st、2nd、4th、6th day in anti-MIF Ab group and saline group respectively, observing the general situations and scoring Disease Activity Index (DAI) of these mice every day. Mice were killed on the eighth day and evaluated with scores of macroscopic and microscopic damage in colonic tissue under the light microscope on HE staining, meanwhile, the expression of inflammatory cytokines, such as MIF、TNF-α and IL-6 were observed by utilizing immunohistochemical staining and the expression of NF-κB p65 mRNA were also detected by Real-Time PCR in colonic mucosa tissue. Results:Compared with normal group, the degree of colonic inflammation were significantly severe in the other groups, the expression levels of inflammatory cytokines such as MIF、TNF-α and IL-6 were remarkably increased (p<0.05); compared with model group, the degree of colonic mucosa damage alleviated in intervention group, anti-MIF Ab could obviously down-regulate the expressions inflammatory cytokins such as MIF、TNF-α and IL-6 (p<0.05), but no significant difference was observed between saline group and model group (p>0.05). In addition, anti-MIF Ab could apparently inhibit the expression NF-κB p65 mRNA in colonic tissue. Conclusions:Anti-MIF Ab has a certain protective effect on mice with ulcerative colitis, the inhibition of NF-κB signal pathway and the decreasing of inflammatory cytokines may involve in the protective process.