Bone Marrow Stromal Cells (BMSCs) Attenuate Apoptosis Partly Via Mitochondrial Transfer In Spinal Cord Iniury Rats

Objectives:We will explore the effects of bone marrow stromal cells (BMSCs) transplanted in spinal cord after spinal cord injury(SCI), and its protential mechanism.Mothods:In vivo, spinal cord injury (SCI) on SD rats is in established improving by Allen’simpact method. The efficiency of BMSCs is evaluated by the fluorescence of Dil in spinal cord. The motor function of rats was assessed by BBB scores. In vitro, primary BMSCs were cultured and characterization. Culture Oxygen-Glucose Deprivation (OGD) model of VSC4.1 motor neurons was established by sealed hypoxic GENbag. To established the system of co-culture beween BMSCs and VSC4.1 motor neurons. The apoptosis of VSC4.1 motor neurons were analysised by Flow cytometry assay and wright staining. The cell viability of VSC4.1 motor neurons after co-culture of with BMSCs-CM was measured by CCK-8. The transplantation of mitochondria will be observed between BMSCs and VSC4.1 motor neurons.Results:In vivo, the fluorescence of GFP suggested that BMSCs were transplanted successfully into the spinal cord. The locomotor function of rats was evaluated by BBB score better in BMSCs transplanted group.In vitro, co-culture with BMSCs inhibited OGD-induced apoptosis in motor neuron. Co-culture with BMSCs-CM promoted survival of post-OGD VSC4.1 motor neurons. Mitochondria transfer from BMSCs to motor neuron after co-culture.Conclusion:BMSCs may contribute to the functional restoration from the SCI rats and inhibited OGD-induced neurons apoptosis, which may be associated with microparticles secreted by BMSCs.