The Activity Of Hawthorn Extract And The Identified Related Compounds

This experiment is to understand and study the hawthorn with antioxidant and fall blood sugar active compounds in specific area, and determine the types and structures of compounds.With petroleum ether, dichloromethane, ethyl acetate and n-butanol of hawthorn extracts of ethanol extraction, by petroleum ether, dichloromethane, ethyl acetate and n-butanol extracts, and calculate the extraction yield of each layer extracts.Of 4 kinds of extracts of antioxidant activity screening, live and fall blood sugar and petroleum ether and ethyl acetate extract of the analysis of the thin layer chromatography and column chromatography separation and recycling semipreparative liquid chromatography for the separation and purification of further extract. Finally, the separation of the compound antioxidant activity and fall blood sugar to experiment on.Results show that n-butyl alcohol extract layer of withdrawal rate is highest, at 2.43%;Antioxidant activity screening results show that the ethyl acetate layer best antioxidant activity of extracts, petroleum ether layer extracts antioxidant activity were also well; Fall blood sugar activity screening results show that the petroleum ether and ethyl acetate extract has good activity; Of petroleum ether and ethyl acetate extracts of separation, we got 7monomer compounds, respectively is double coniferyl alcohol dehydrogenation, rosin alcohol,vanillic acid, vanillin, ursolic acid, hexadecanoic acid and hexadecanoic acid glyceride.Through oxidation of seven compounds and fall blood sugar active experiment, found that ursolic acid has strong antioxidant activity and fall blood sugar, vanillic acid also has certain antioxidant activity. Keywords: hawthorn. Antioxidant activity. Fall blood sugar activity; Thin layer chromatographyWe can find from the hawthorn extract is a good antioxidant activity and fall blood sugar, and with good resistance to oxidation and fall blood sugar active compounds mainly exist in the petroleum ether and ethyl acetate parts, antioxidant activity is one of the best ursolic acid and vanillic acid, fall blood sugar activity is one of the best ursolic acid.