| Objective:pregnancy pregnant rats low protein feed to establish all the intrauterine retardation (intrauterine growth retardation, IUGR) newborn rat model. For after the birth of IUGR in newborn rats given different protein feed, monitoring the pancreas tissues MAFA and MUNC13-1 protein expression in,From the aspects of molecular biology analysis optimization to improve feeding forward the mechanism of action of the metabolic syndrome.Methods:select 20 female mice, the body quality of 215-235 g,80-80 days, day age there is no history of mating;Males only 10, body quality of 250-300 g, Female determine single cage feeding after conception, use the entire pregnancy diet low in protein (6.51%) and restricted diet IUGR animal experimental model is established. SD rat pregnancy can be divided into IUGR group and normal diet control group, respectively give low protein feed and feed standard. After childbirth pregnant rats intrauterine retarded standard IUGR group newborn larvae mice were randomly divided into three groups:IUGR standard protein, IUGR low-protein group, IUGR high-protein group, after giving birth mothers were to be low standard feed, protein feed, protein feed for three weeks, made wang rat after weaning feed standard until the end of the experiment. Normal diet control group rats to standard feed 3 weeks, after weaning rat feed to standard until the end of the experiment Balance in 12 hours after birth,4 weeks after birth,8 weeks after birth weight, three time points after anesthesia scale pancreatic tissue weight, low temperature preservation. Rats with ELISA kit, respectively measured between groups of three time points MafA protein and protein levels MUNC 13-1.Results using SPSS13.0 statistics software to carry on the analysis, P< 0.05 said the difference was statistically significant.Results:1. Stillbirths in IUGR group:rats stillbirth rate was 13.6%(14/104), normal diet control seed rat stillbirth rate was 4.8%(3/62), the difference was statistically significant (P< 0.05).2. Born IUGR group and normal diet group of young rat body quality (g) were 6.6.61+/-0.51,5.08+/-0.41, the difference was statistically significant (P< 0.05)3. The incidence of IUGR IUGR group:the incidence of IUGR rat was 79%(84/104), normal diet group wang incidence of IUGR rat was 6.4% (4/62), the difference was statistically significant (P< 0.05).4. Mass (g):born IUGR group of young mice and the body quality of diet control group were5.48+/-0.48, 6.89+/-0.39, is similar between the two groups have significant statistical significance (P< 0.05).4 weeks in normal diet group, IUGR standard protein, IUGR low-protein group, IUGR high-protein group quality are:74.27+/-5.165,5.165, 29.65+/-3.636,3.6364.792 mm+/-8.143; Normal diet control group and IUGR standard protein group no difference of quality, body quality of IUGR low-protein group were lower than normal diet control, IUGR standard protein groups and IUGR high-protein group, Control group and normal diet, IUGR standards body quality is lower than the IUGR high-protein group protein group.At 8 weeks, IUGR standard protein group, normal diet control group IUGR low-protein group, high IUGR egg mass are:160.4+/-13.46,146.1+/-12.16,12.16,158.810.08 mm,14.98 mm IUGR body quality protein group is lower than the normal control group and IUGR high-protein diet, IUGR standard protein body quality higher than that of IUGR low-protein group, control group and normal diet than IUGR low-protein group, normal diet control group no difference with IUGR high-protein diet group.5. The pancreas weight (g):within 12 hours after birth weight of IUGR group and normal control group, respectively:0.0149+/-0.006,0.01320.004 mm; IUGR pancreas weight is lower than the normal control group, P< 0.05, statistical differences. Normal diet control group 4 weeks after birth, IUGR standard protein group, IUGR low-protein group, IUGR high-protein pancreas weight are:0.2691+/-0.040,0.3382 +/-0.054,0.1195+/-0.027,0.4018 0.034 mm; IUGR low-protein group pancreas weight under standard of IUGR protein, IUGR high-protein group and normal control group, P< 0.05 statistical differences, IUGR standard protein and IUGR high-protein group than the normal control group, IUGR standard protein below IUGR high-protein group.8 weeks after birth, IUGR standard protein diet control group group, IUGR low-protein group, IUGR high-protein pancreas weight are:0.448+/-0.055,0.438+/-0.043,0.387+/-0.070,0.494 0.100 mm, pancreas weight no significant differences between groups (P>0.05).0 weeks in normal control group with IUGR group in the pancreas MafA protein concentration has no obvious difference (P> 0.05);4 weeks in IUGR low-protein group with IUGR high-protein and IUGR standard statistical differences compare MafA protein concentration in the protein group, IUGR low-protein group concentration is lower than the IUGR high-protein and IUGR standard protein group (P< 0.05);IUGR low-protein group compared with normal control group no significant statistical difference (P> 0.05);IUGR standard protein with IUGR high-protein group was no significant statistical difference (P> 0.05);At 8 weeks IUGR low-protein group MafA protein concentration under standard of IUGR high-protein group and IUGR group, ther are statistically significant difference (p< 0.05);IUGR low-protein group and noaanal control group no significant difference (P> 0.05);IUGR high-protein and IUGR standard protein group MafA protein concentration is greater than the normal control group (P< 0.05), IUGR high-protein MafA protein concentration is greater than the standard of IUGR protein group, the difference was statistically significant (P< 0.05).7 different time points, and group comparison MUNC13-1 protein concentration: 0 weeks MUNC13 IUGR group 1 protein content is lower than that of normal control group, difference has statistical significance (P< 0.05); At 4 weeks IUGR high-protein feed protein concentration is greater than the IUGR group MUNC13-1 low protein feed significantly statistical differences (P< 0.05), IUGR group compared with normal control group has no significant statistical difference (P>0.05);At 8 weeks IUGR low-protein group MUNC13-1 protein concentration higher than that of IUGR high-protein, IUGR standard protein group and normal control group (P< 0.05), IUGR high-protein and IUGR standard protein group compared with normal control group no significant difference (P> 0.05).7 different time points, and group comparison MUNC13-1 protein concentration:0 weeks MUNC13 IUGR group 1 protein content is lower than that of normal control group, difference has statistical significance (P< 0.05);4 weeks in IUGR MUNC13-1 protein concentration has no obvious difference between groups (P> 0.05), IUGR group compared with normal control group has no significant statistical difference (P> 0.05);At 8 weeks IUGR low-protein group MUNC13-1 protein concentration higher than that of IUGR high-protein, IUGR standard protein group and normal control group (P< 0.05), IUGR high-protein and IUGR standard protein group compared with normal control group no significant difference (P> 0.05).Conclusions:.1. Give a high-protein diet, early after birth can make-chow IUGR rat complete catch-up growth in 4 weeks after birth.2. The IUGR standard protein feed with IUGR high-protein feed group after early reinforce the feeding, higher MafA protein, which may improve insulin resistance.But under the early low protein feed, which can lead to MafA protein decreased, which may be low protein feed can increase the long-term one of risk factors of metabolic syndrome.3. Early in the low protein feed MUNC13-1 protein in the pancreatic tissue under the abnormal secretion, the standard protein and protein intervention after the normal protein secretion, it may be early strengthening feed on one of the long-term mechanism of action of the metabolic syndrome. |
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