| Mycoplasma pneumoniae is the main pathogen of mycoplasma pneumonia in children and also a common pathogens of respiratory tract infection in children. Clinical commonly used serological detection method for testing with other common respiratory pathogens such as Legionella bacteria (LP);Q Coxiella burnetii (CB);Chlamydia pneumonia (CP); Adenovirus (ADV); Respiratory syncytial virus (RSV); Influenza A virus (state-run Influenza A virus get);B (state-run Influenza B virus get);Parainfluenza virus (HPIVs) detection.Objective:Current molecular biology detection technology has been widely used in clinical diagnosis, the PCR technology was very mature than before, LAMP as a new molecular biological detection technology has been moved 20 years. This paper I want to use LAMP method to detect mycoplasma pneumoniae, and compared with PCR technology, by the way of the discussing of the two methods characteristics and the difference of specificity and sensitivity, to get some ideas about what’s the better methods of the molecular biology detection technology in testing MP.Methods:Use professional software design 4 LAMP primers to detect mycoplasma pneumonia and establish the LAMP methods; we use the same primers to test LAMP methods we established with Legionella and Adenovirus at the same time to verify the LAMP specificity. Use the same specificity sample by the way of the LAMP and the PCR reaction to comparing the sensitivity of the two methods.Conclusion:Results show that the LAMP has strong specificity, LAMP sensitivity in the detection of mycoplasma pneumonia was better than PCR. Experiments results that LAMP technology can detect diluted 10-4 samples, and the PCR can detect the diluted 10-2 samples.Objective:Mycoplasma pneumoniae(MP) is one of the common cause of community-acquired pneumonia(CAP) in child and other respiratory tract infections, it also can attacked all the year. The most common manifestations of respiratory symptoms include fever, cough, asthma, etc. it also can cause variety extrapulmonary complications, such as encephalitis, myocarditis, skin damage, urinary system and digestive system damages, a few children with various complications attacked at the same time, if have not timely cured, severe cases can be life-threatening. This paper collected epidemiological characteristics of MP infection during 2014, I want to provide scientific dates for clinical early diagnosis and reasonable treatment.Methods:Patients who were diagnosed as pneumonia in hospitalized children from 2014 were enrolled. We have collected 3256 cases and with Indirect immunofluorescence technique (Indirect immunofluorescence assay, IIFA) to detect MP antibody specificity MP-IgM.Results:from the 3256 serum cases we get positive rate was 10.6%in spring, summer is 14.2%, autumn was 25.8%,35.1%in winter. The average incidence was 21.4%, by the way of the software SPSS 17.0 analysis dates we can learn that the four seasons have statistical significance (P< 0.05), for the dates of the four season we knows that the fall and winter was higher than other seasons, the difference is statistically significant.< 1 years positive rate is 8%; 1-3 years positive rate is 13%; 3-6 years positive rate is 21%; 6 years the positive rate is 22%. By the way of the SPSS 17.0 (P< 0.05) we can get the data was that between different age has statistically significant, which the age of 3-6 is significantly higher than other age groups has statistically significant, the difference is statistically significant.Conclusion:Mycoplasma pneumoniae occurs mostly in children of school age among 3-6 years, autumn and winter season with higher rates than other seasons. |
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