Study Of The Relationship Between Negative Costimulatory Molecule B7-H1 And The Chemotherapeutic Sensitivity In Breast Cancer

Objectives: To investigate the relationship between B7-H1 and the chemotherapeutic sensitivity in breast cancer cell, establish breast cancer cell line with B7-H1 over-expression and observe the level of proliferation and apoptosis under chemotherapy treatment with epirubicin and docetaxel.Methods:Establish breast cancer cell line with MCF-7 /B7-H1+ and MCF-7/mock cell line via Lentivirus infect human breast cancer cell line MCF-7. Detect the established cell line with real-time PCR,flow cytometry and western-blot.Cell proliferation and apoptosis after chemotherapy treatment with epirubicin and docetaxel were analyzed by CCK8 and annexin V/PI staining kit.Results: MCF-7 /B7-H1+ cell line and MCF-7/mock cell line were successfully established. MCF-7 /B7-H1+ breast cancer cell have greater ability of proliferation and lower lever of cell apoptosis compared with the MCF-7/mock cell line. Conclusions:Upregulate the expression of B7-H1 on MCF-7 cells reduce the effect of proliferation inhibition and apoptosis caused by chemotherapy. MCF-7 /B7-H1+ breast cancer cell could help the tumor cells survive from the apoptosis which induced by epirubicin and docetaxel.B7-H1 could enhance the cancer cell proliferation and the ability of apoptosis resistance. Negative costimulatory molecule B7-H1 is related with chemotherapeutic sensitivity in breast cancer.Upregulate the expression of B7-H1 can reduce the ability of chemotherapeutic sensitivity in breast cancer.