The Effect Of Turmeric Volatile Oil On The Biological Effects Of SH-SY5Y Cells In Neuroblastoma Cells And Its Effect On The Sensitivity To Chemotherapy

BackgroundNeuroblastoma(NB) is a childhood the most common solid tumors [1], the incidence rate in children with malignant tumors among the four [2], derived from the embryonic neural crest cells, composed of undifferentiated sympathetic nerve cells, because of its high degree of malignancy, transfer early, high mortality and poor prognosis known as the king of children with cancer[3]. Clinical rely mainly on surgery, radiotherapy, chemotherapy and other comprehensive treatment method. In order to improve the treatment and prevention of recurrence and to ensure the quality of life of children, in the treatment of NB, medicinal chemistry due to effects on tumor has become indispensable, but clinical practice proved that chemical medicine exists different degree of side effects and drug resistance. Therefore, domestic and foreign scholars gradually the research direction to the biology of NB, genetic characteristics and pathogenesis research, to find a new method for tumor therapy, improve the patient cure rate and improve the long-term prognosis of childhood, and has important clinical value[4,5]. So the selection of God by neuroblastoma SH-SY5 Y cell line to study of the volatile oil from Curcuma longa(turmeric of volatile oil TVO) antitumor effect and the anti NB effect of the combined with cisplatin(DDP) was studied.This paper is divided into two parts to discuss: the first part describes the turmeric volatile oil of neural neuroblastoma SH-SY5 Y cells proliferation and invasiveness of the influence; the second part of turmeric essential oil combined with small dose of cisplatin on neuroblastoma SH-SY5 Y cells inhibition.Partâ… :Effect of proliferation and invasiveness by turmeric volatile oil on neuroblastoma cell line SH-SY5YObjectives To investigate the effect of apoptosis,proliferation,migration and invasiveness by turmeric volatile oil on human neuroblastoma cell line SH-SY5 Y.Methods 1.SH-SY5 Y cells were incubated with different concentrations(1.25-160 mg/L) of TVO in vitro. Then cell survival rate was measured by MTT assay. 2.The effect of 160 mg/L TVO on cell migration was assessed by cell scuffing test. 3.Invasive ability of SH-SY5 Y was detected by Transwell test. 4.Apoptosis of cells was detected observed by flow cytometry assay.Results Survival rate of SH-SY5 Y cells was decreased and apoptisis rate was abated with elevated TVO concentration and lengthened cultivation time(P<0.01);level of SH-SY5 Y cell migration was lower than that in control group after being cultured with 160 mg/L TVO solution for 12,24 and 48h(P<0.01). With the increase of TVO concentration, the invasion ability of SH-SY5 Y cells gradually decreased(P<0.01), and the invasive force and cisplatin had no obvious difference when the concentration of drug reached 160 mg/L.Flow cytometry detection showed that the combined group, DDP group, TVO group and the control group compared to the difference was statistically significant(P< 0.01); different concentrations of TVO combined with a concentration of DDP and separate the concentration of DDP group compared to the difference has statistical significance(P< 0.01), and total cell apoptosis rate increased with increasing concentration of TVO.Conclusion TVO combined with small dose of DDP can enhanced the inhibitory effect of cisplatin on the proliferation of SH-SY5 Y cells, and have obvious synergistic effect, TVO on growth mechanism of sensitization may be is TVO assist DDP specific activation of Caspase-3 to induce apoptosis in SH-SY5 Y cells.Partâ…¡:The Inhibitory effects of TVO in Combination with low dose of Cisplatin on neuroblastoma cell line SH-SY5YObjectives: To discuss the effects of combination of TVO and low dose of cisplatin on SH-SY5 Y cells,to enhance the anti-tumor effect of drug in vitro, and to explore the molecular mechanism.Methods : Respectively with TVO, DDP, and combination processing in SH-SY5 Y cells. MTT assay was used to determine the treatment of cell growth inhibition rate and IC50 value; according to the MTT assay results set up a blank control group, TVO group, DDP group and combination group,Inverted microscope was employed to observe morphologic changes, and the expression of Caspase-3 detection reagent kit for detecting SH-SY5 Y cells caspase-3 enzyme.Results : Separate TVO and DDP group with their concentration increased the proliferation rate of SH-SY5 Y cells increased gradually(P<0.01); different concentrations of TVO combined with low dose of DDP(0.625,1.25,2.5,5g/ml) in SH-SY5 Y cells after 48 h proliferation inhibition rate increased gradually, and the difference between the TVO alone group, DDP alone group and control group was significant(P<0.01); Under the inverted microscope DDP group and combination group the death is obvious, especially in the combination group was more obvious than that of blank control group and TVO group,cells grew slowly and the number of rare.TVO combined with DDP(0.625,1.25,2.5g/ml) group and TVO group separately Caspase-3 activity with the increase of drug concentration with enzyme activity enhanced(P<0.01); DDP alone group and control group Caspase-3 activity had no significant difference(P > 0.05); with the combined group compared with DDP group the activity of Caspase-3 water level difference Statistical significance(P<0.01).Conclusion: TVO combined with low dose of DDP can enhance the proliferation inhibition of SH-SY5 Y cell line, and it has obvious synergistic effect, and the mechanism of TVO on the sensitivity of TVO may be closely related to the activation of Caspase-3.