To Investigate The Mechanism Of Combination Warming Yang With Reducing Urine To Treat The Overactive Bladder Through Nerve-ICC-smooth Muscle Network

ObjectiveOveractive bladder (OAB) is a syndrome characterized by symptoms of urgency, its significantly affect patients’daily life and social activities, become a major disease in people. Neural network as a new thought of OAB nerve the ICC-smooth muscle of one of the causes, research focus increasingly caught the attention of the researchers. Suoquan pill effect for kidney urine, solid check off. Shenqi Wan can encourage the kidney of yang qi. Clinical feedback shows that Suoquan Wan and Shenqi Wan has good curative effect on treatment of OAB, method of this topic in kidney shrinkage urine neural network as the breakthrough point, ICC-smooth muscle research kidney urine method on the mechanism of action of OAB disease.MethodsIn this study the overactive bladder model as the research model. Divided into normal group, model group, positive group, the Suoquan pill group, the Shenqi pills group and Suoquan pill combination Shenqi wan group. With frequency of urination, urine output, urine dynamics parameters and in vitro muscle function to preliminary discussion method of kidney shrinkage urine bladder disease treatment effect of excessive activity. By organizing immunof luorescence observations bladder ICC, acetyl choline can nerve (VAChT) and substance P peptide can vary in the number of nerve, and transmission electron microscope integral bladder neural network structure-ICC-smooth muscle, observe whether kidney shrinkage method of urine is through neural network-ICC-smooth muscle shrinkage effect of urine. By organizing immunofluorescence double standard ICC cells and M3, P2X2 receptor pressure type of expression, as well as using the fluorescent quantitative PCR method, western blotting method to detect the ICC-smooth muscle protein network gap junction Cx43 mRNA and protein expression, from the bladder nerve-the ICC -smooth network study the effect of the kidney shrinkage urine method of signal transduction in the mechanism of the bladder. With bladder neural network the ICC-smooth muscle as a breakthrough point, to explore method of kidney shrinkage urine bladder disease mechanism of excessive activity.Results1、The model group of the number of guinea pig urine significantly increased, bladder leakage point pressure increase (BLPP) and the maximum bladder pressure (MVP) and the effect of residual urine volume (PVR) (P<0.05); Model group significantly increased the bladder muscle strips of agonist stimulation sensitivity, to caused by KC1 agonist and Carbachol contraction has significantly increased (P<0.05); ICC can significantly increase and nerve can significantly decrease of the relative expression (P<0.05, P<0.01), model group bladder neural network of connections between ICC-smooth muscle more closely; The ICC nerve receptors on cells M3 and P2X5 expression after building elevated significantly (P<0.05), significantly increased the mRNA and protein expression of Cx43 (P<0.01).2、The treatment of kidney shrinkage urine method in the OAB role: Tolterodine, Suoquan pill or the treatment of kidney shrinkage urine method treatment can reduce the number of OAB model of guinea pig bladder urination (P<0.05), Tolterodine, Suoquan pill, kidney shrinkage urine method has different rate reduced BLPP, MVP and the role of PVR (P<0.05). Tolterodine, Suoquan pill and kidney shrinkage urine method are significantly lower because of different level caused by KCL agonist muscle contraction effect (P<0.05), and in Carbachol contraction function under the influence of different concentrations were significantly lower (P<0.05).3、Method of kidney shrinkage urine OAB neural network structure-the ICC-smooth muscle of guinea pig bladder:immunofluorescence results show that the given Suoquan pills, kidney and urine kidney shrinkage method have different degree to reduce the ICC cells and increase nerve fibers relative expression of substance P, which the effect is the most significant kidney shrinkage urine group (P< 0.05). Transmission electron microscopy (TEM) shows that Tolterodine were given to the guinea pigs, Suoquan pills, kidney pills or kidney shrinkage method of urine after treatment, to some extent improve nerve-ICC-the relationship between structure of smooth muscle.4、The method of kidney shrinkage urine OAB guinea pig bladder neural network signaling the ICC-smooth muscle:the influence of the nerve receptors and ICC immunofluorescence double standard, according to the results of kidney shrinkage method of urine after treatment, the ICC M3 nerve receptors on cells express declined significantly (P<0.05); Kidney shrinkage method after the intervention, urine bladder Cx43mRNA gap junction protein and protein expression was significantly decreased (P<0.01), the method of filling kidney shrinkage urine can achieve by reducing the impulse signal transmission and adjusting bladder function.Conclusion1. The model group can increase micturition frequency model group, unstable with smooth muscle contraction, and the sensitivity of agonist accompanied by significant increases, on behalf of the building successfully. The bladder ICC number increases, on behalf of OAB changes with the number of ICC.2. The combination warming Yang with reducing urine group obviously improve bladder guinea pigs micturition number excessive activity, can reduce the bladder leakage point pressure (BLPP), maximum urinary bladder pressure (MVP) and residual urine volume (PVR), improve the bladder muscle tension activities, preliminary evidence that kidney shrinkage overactive bladder of bladder urine method can improve pathological state.3. The model group obviously increase the number of ICC and obviously increase the number of nerve fibers and tem results show that the network of neural-the ICC-smooth muscle closer together. The Suoquan pill combination shenqi Wan treatment can improve the bladder nerve-ICC-smooth muscle network connection, after dosing drugs can be decreased by ICC and the number of nerve fibers which have the effect of kidney shrinkage urine.4. The combination warming Yang with reducing urine group can reduce the number of nerve receptors, reduce the amount of gap junction protein expression, improve OAB guinea pig bladder neural network signaling ICC-smooth muscle. Explain the combination warming Yang with reducing urine by improving the connection between the network of neural-ICC-smooth muscle kidney shrinkage of urine.