Studies On The Chronic Lung Injury And Its Mechanism In The F344 Rats Induced By Different Coal Of Xuanwei Yunnan

Objective:It is to explore the lung injury and the changes of cytokines of F344 rats which inhale fine particulate matter which is released by coal combusting of Xuanwei.Methods:F344 rats which inhale fine particulate matter released from coal combusting are selected as the experiment object. According to the incidence of lung cancer, the F344 rats are divided into three groups:coal of high incidence area group, coal of low incidence area group, blank control group. According to the time of inhaling, coal of high incidence area group is divided into 2 hours group and 1 hour group,and the same as coal of low incidence area group. The inhaling time is 4 weeks,8 weeks,12 weeks,16 weeks,20 weeks and 24 weeks. The F344 rats will be killed when they reach the time, and getting the lung tissue and serum of the rats. The lung tissue of rats is made into pathological section which is used to observe the lung injury of the rats under the electron microscope. ELISA method is used to measure the levels of interleukin-6, interleukin-8, tumor necrosis factor alpha and interleukin-1 beta in serum of each group of rats. SPSS 17.0 statistical software is used to analyze the concentration of cytokines in serum of each group of rats,and one-way ANOVA is used to analyze the result.Results:1 The lung injury in F344 rats induced by fine particle matter released in different coal of in Xuanwei1.1 General situation of F344 rats during the experiment During the experiment, none is dead. As time increasing the amount of activity and food intaking of the rats in coal of high incidence area 2 hours group and 1 hours group is decreasing, but the change of water intaking is not obviously, and the hair of the rats becomes yellow and dull, and the spirit is poor, but no significant changing in body weight. The same as the coal of low incidence area 2 hours group and 1 hours group rats. In the blank control group, there is no significant change in the mental state, and diet,and activity and body weight of the rats.1.2 The lung injury of F344 rats under the light microscope4 weeks,the bronchial tube wall of coal of high incidence area 2 hours group rats is slightly thickened, and the inflammatory cell is seen around the airway wall, a tendency to expand with the alveolar cavity. With time increasing, and the damage of bronchial mucosa is more and more seriously. Bronchial wall which is surrounded by a large number of inflammatory cell is thicking obviously, and alveolar wall is more and more thinning and rupturing into bullae.16weeks, one rat have got lung cancer.20 weeks,two rats have got lung cancer.24 weeks, three rats have got lung cancer; The same as the coal of low incidence area 2 hours group,but there is only one getting lung cancer in 16 weeks. The same as the coal of high incidence area 1 hours group and coal of low incidence area 1 hours group,but there is no one getting lung cancer. The lung tissue of the rats in the blank control group is no change, and the structure is completely, and there is no inflammatory cell infiltrating.2 Changes of cytokines in serum of F344 rats2.1 Changes of IL-6 in serum of F344 rats4 weeks,12 weeks,16 weeks,24 weeks, there is no obviously difference in the concentration of IL-6 in serum of rats among the coal of high incidence area 2 hours group and coal of low incidence area 2 hours group and blank control group, the difference does not have statistically significant.8 weeks,20 weeks, there is obviously difference in the concentration of IL-6 in serum of rats among the coal of high incidence area 2 hours group and coal of low incidence area 2 hours group and blank control group, the difference has statistically significant.There is no obviously difference in the concentration of IL-6 in serum of rats among 4 weeks and 8 weeks and 12 weeks and 16 weeks and 20 weeks and 24 weeks in the coal of high incidence area 2 hours group, and the difference does not have statistically significant.There is no obviously difference in the concentration of IL-6 in serum of rats among 4 weeks and 8 weeks and 12 weeks and 16 weeks and 20 weeks and 24 weeks in the coal of low incidence area 2 hours group, and the difference does not have statistically significant.2.2 Changes of IL-8 in serum of F344 rats4 weeks,20 weeks,24 weeks, there is obviously difference in the concentration of IL-8 in serum of rats among the coal of high incidence area 2 hours group and coal of low incidence area 2 hours group and blank control group, the difference has statistically significant.8 weeks,12 weeks,16 weeks, there is no obviously difference in the concentration of IL-8 in serum of rats among the coal of high incidence area 2 hours group and coal of low incidence area 2 hours group and blank control group, the difference does not have statistically significant.There is obviously difference in the concentration of IL-8 in serum of rats among 4 weeks and 8 weeks and 12 weeks and 16 weeks and 20 weeks and 24 weeks in the coal of high incidence area 2 hours group, and the difference has statistically significant.There is no obviously difference in the concentration of IL-8 in serum of rats among 4 weeks and 8 weeks and 12 weeks and 16 weeks and 20 weeks and 24 weeks in the coal of low incidence area 2 hours group, and the difference does not have statistically significant.2.3 Changes of TNF-a in serum of F344 rats4 weeks, there is obviously difference in the concentration of TNF-a in serum of rats among the coal of high incidence area 2 hours group and coal of low incidence area 2 hours group and blank control group, the difference has statistically significant.8 weeks,12 weeks,16 weeks,20 weeks,24 weeks, there is no obviously difference in the concentration of TNF-a in serum of rats among the coal of high incidence area 2 hours group and coal of low incidence area 2 hours group and blank control group, and the difference does not have statistically significant.There is no obviously difference in the concentration of TNF-α in serum of rats among 4 weeks and 8 weeks and 12 weeks and 16 weeks and 20 weeks and 24 weeks in the coal of high incidence area 2 hours group, and the difference does not have statistically significant.There is no obviously difference in the concentration of TNF-α in serum of rats among 4 weeks and 8 weeks and 12 weeks and 16 weeks and 20 weeks and 24 weeks in the coal of low incidence area 2 hours group, and the difference does not have statistically significant.2.4 Changes of IL-1β in serum of F344 rats4 weeks,8 weeks,12 weeks,16 weeks,20 weeks,24 weeks, there is no obviously difference in the concentration of IL-1β in serum of rats among the coal of high incidence area 2 hours group and coal of low incidence area 2 hours group and blank control group, and the difference does not have statistically significant.There is obviously difference in the concentration of IL-1β in serum of rats among 4 weeks and 8 weeks and 12 weeks and 16 weeks and 20 weeks and 24 weeks in the coal of high incidence area 2 hours group, and the difference has statistically significant.There is no obviously difference in the concentration of IL-1β in serum of rats among 4 weeks and 8 weeks and 12 weeks and 16 weeks and 20 weeks and 24 weeks in the coal of low incidence area 2 hours group, and the difference does not have statistically significant.Conclusion:1 The fine particles matter which is released by coal combusting of Xuanwei plays an important role in the pathogenesis of lung cancer in Xuanwei.2 The fine particulate matter which causes lung injury may be one of the main approaches to induce lung cancer, but the cytokine related inflammatory response is not the main reason to induce lung cancer, and other mechanism may be involved in the occurrence of Xuanwei lung cancer, and there is an important significance to explore the pathogenesis of Xuanwei lung cancer.