The Level And Clinical Significance Of Treg,MDSC And TGF-β In The Peripheral Blood Of Patients With Bladder Urothelial Carcinoma

Objective:Investigate the level of regulatory T cell(Treg), MDSC and TGF-β in the peripheral blood of bladder urothelial carcinoma patients and health group, to compare the difference between the bladder urothelial carcinoma group and health group, then discuss its relationships with clinical pathological characteristic of bladder urothelial carcinoma. The correlations among Treg, MDSC and TGF-β were also analysed to investigate their roles and intractions in bladder urothelial carcinoma.Methods:1. Flow cytometry was used to investigate the prevalence of Treg in the peripheral blood of 114 patients with urothelial bladder cancer and 30 healthy controls, the difference between the bladder urothelial carcinoma group and health group was analyzed, The relationships of Treg level with clinicopathological factors was analyzed statistically.2. Flow cytometry was used to investigate the prevalence of MDSC in the peripheral blood of 57 patients with urothelial bladder cancer and 30 healthy controls, the difference between the bladder urothelial carcinoma group and health group was analyzed, The relationships of Treg level with clinicopathological factors was analyzed statistically.3. The expression of TGF-β in the peripheral blood of 114 bladder urothelial carcinoma patients and 30 healthy controls were detected by enzyme-linked immunoassay, the difference between the bladder urothelial carcinoma group and health group was analyzed, the relationship of TGF-β expression with clinicopathological factors was analyzed statistically.4. Then the relationships among Treg, MDSC and TGF-β were analyzed to investigate their interations in the bladder urothelial carcinoma.5. All dates were analyed with SPSS17.0. Measurement date results was given as means ±SEM(x ± s). Statistical analysis was performed by SPSS17.0 software,correlation analysis by Pearson correlation analysis. It was considered statistically significant when P vule was less than 0.05.Results:1.The level of Treg in the peripheral blood of patients with bladder urothelial carcinoma was significantly increased(P<0.01), compared with the health controls.The level of Treg in high histologic grade was higher than low histologic grade, the difference was statistically significant(P<0.01), it were not correlated with age,gender, tumor diameters, tumor number, clinical stage or tumor recurrence(P>0.05).2.The level of MDSC in the peripheral blood of patients with bladder cancer was significantly increased(P<0.01), compared with the health controls. The prevalence of MDSC was associated with tumor pathological grade and tumor number(P<0.05),but not with other clinicopathological factors.3.TGF-β in the peripheral blood of patients with bladder cancer was significantly increased compared with the health controls(P<0.01), the difference of TGF-βexpression level between low histologic grade and high histologic grade was statistically significant(P<0.05), but not correlated with the other factors(P>0.05).4.Significant positive correlation between circulating MDSC and Treg was observed(r = 0.433, P<0. 01), The variance of TGF-β was significantly positively correlated with Treg and MDSC(r = 0.515, P<0. 01; r =0.353, P<0. 01).Conclusions:1.The revalence of Treg, MDSC and TGF-β were obviously increased in the peripheral blood compared with health controls, suggesting an immune suppression in the patients with urothelial bladder cancer.2.The difference expression level of Treg, MDSC and TGF-β between lowhistologic grade and high histologic grade was statistically significant, while the level of them were higher in high histlogic grade tumor group than the low histologic group, which may suggest that immune suppression is closely related with bladder urothelial carcinoma.3. Positive correlationships among Treg, MDSC and TGF-β may suggest that the immunology factors have complicated interaction with each other.,which commonely affect the occurance,development and prognosis of tumor.