The Effects Of Methyleugenol On MUC5AC And AQP1、AQP5 In Nasal Mucosa Of Rats With The Allergic Rhinitis

Objective: To observe the effect of methyleugenol on MUC5 AC and AQP1, AQP5 in nasal mucosa of rats with the allergic rhinitis(AR).Methods: AR models were established with Wistar rats by ovalbumin-induction(OVA).The rats were randomly divided into normal control group, model group, loratadine group and methyleugenol group(10 mg/kg group、20 mg/kg group and 40 mg/kg group),and each group was administrated with the corresponding drugs. The nasal mucosa of the rats was obtained in 1 week, 2 weeks, 4 weeks and 6 weeks, and the expression of MUC5 AC protein was detected by immunohistochemistry and the quantity of MUC5 AC mRNA was tested by real-time fluorescence quota PCR(RT-PCR).Results: Comparing with the model group, the percentage of positive expression of MUC5 AC protein and the relative quantity of MUC5 AC mRNA in 20 mg/kg and 40 mg/kg groups were significantly decreased after 2 and 4 weeks’ administration and the difference was statistically significant. The percentage of positive expression of MUC5 AC protein and the relative quantity of MUC5 AC mRNA in 10 mg/kg group were no significant difference in the whole process of administration. The percentage of positive expression of MUC5 AC protein and the relative quantity of MUC5 AC mRNA in loratadine group were significantly decreased after 1 week’s administration and the difference was statistically significant. Comparing with the loratadine group, the percentage of positive expression of MUC5 AC protein in 20 mg/kg group after 6 weeks’ administration were in higher level, and the percentage of positive expression of MUC5 AC protein and the relative quantity of MUC5 AC mRNA in 40 mg/kg group after 6 week of administration were no difference. Comparing with the loratadine group, the relative quantity of MUC5 AC mRNA in 20 mg/kg and 40 mg/kg group after 4 weeks administration were no difference. Comparing with the model group, the percentage of positive expression of AQP1 protein and the relative quantity of AQP1 mRNA in 40 mg/kg and 20 mg/kg groups were significantly decreased after 4 and 6 weeks’ administration and the difference was statistically significant. The percentage of positive expression of AQP1 protein and the relative quantity of AQP1 mRNA in 10 mg/kg group were no significant difference in the whole process of administration. The percentage of positive expression of AQP1 protein and the relative quantity of AQP1 mRNA in loratadine group were significantly decreased after 1 week’s administration and the difference was statistically significant. The percentage of positive expression of AQP5 protein and the relative quantity of AQP5 mRNA in 40 mg/kg and 20mg/kg groups were significantly increased after after 2 and 4 weeks’ administration and the difference was statistically significant. The percentage of positive expression of AQP1 protein and the relative quantity of AQP5 mRNA in 10 mg/kg group were no significant difference in the whole process of administration. The percentage of positive expression of AQP5 protein and the relative quantity of AQP5 mRNA in loratadine group were significantly increased after after 1 week’s administration and he difference was statistically significant. Comparing with the loratadine group, the percentage of positive expression of AQP1 protein and the relative quantity of AQP1 mRNA in 20mg/kg group after 6 weeks’ administration were in higher level, and the percentage of positive expression of AQP1 protein and the relative quantity of AQP1 mRNA in 40 mg/kg group after 6 weeks’ administration were no difference. The percentage of positive expression of AQP5 protein and the relative quantity of AQP5 mRNA in 20mg/kg group after 6 weeks’ administration were in lower level, and he percentage of positive expression of AQP5 protein and the relative quantity of AQP5 mRNA in 40 mg/kg group after 6 weeks’ administration were no difference.Conclusion: Methyleugenol can significantly inhibit the expression of MUC5 AC and AQP1, but promote the expression of AQP5 in nasal mucosa of allergic rhinitis rats.