The Inhibited Effect And Regulating Molecular Mechanism Of MiR-424 On The Invasion And Migration In Human Cervical Squamous Cell Carcinoma

Objective:To study the effect and molecular regulating mechanism of mi R-424 on the invasion and migration in human cervical squamous cell carcinoma.Methods: 1. Stem-loop RT-PCR was performed to test the expression of mi RNA; 2.Transwell assays was used to evaluate the ability of cells invasion and migration; the wound healing assays was used to evaluate the ability of cells migration; 3. A bioinformatics search was performed to seek for the predicted targetgenes; the dual luciferase assays were used to further confirm targetgene; 4. The Wnt3 A expression was examined by immunohistochemistry method in squamous cell cervical carcinoma. 5. Real time RT-PCR and Western blot analysis were performed to detect the m RNA transcription level and protein expression.Results: 1.The expression of mi R-424 in the cervical squamous cell cancer tissues was lower than that in the adjacent cancer tissues; 2.The ability of invasive and migrated of Si Ha cells was inhibited after overexpression of mi R-424-5p; 3.Wnt3 A was the downstream targetgene of mi R-424; 4. Immunohistochemistry method showed the positive expression rats of Wnt3 A in cervical cancer tissues and adjacent cancer tissues was 70%; 5. The m RNA level and protein expression of Wnt3 A and MMP-2 were declined after mi R-424-5p overexpression.Conclusion: In the present study, the expression of mi R-424 in the cervical squamous cell cancer tissues was lower than that in the adjacent cancer tissues. mi R-424 regulates the invasion and migration of Si Ha cells via targeting Wnt3 A throughdown-regulating Wnt3 A and MMP-2 expression. This study provides a theoretical and experimental basis for the future reversal of metastasis and novel drug targets development of cervical cancer.