Transfection Efficiency Using PEI-cp Complex For CD133~+ Differently Expressed By Colon Cancer Lines

Objective: To investigate transfect efficiency of CP/DNA at CSC marker CD133+ which is shown to be differently expressed in colon cancer cell lines, and to discuss the relationship between transfect and TR peptide, which is capable to specifically bind to CD133 by targeting CD133+ colon cancer cellsMethods: The expression of CD133+ in four cell lines were divided into 2 groups: the positive colon cell lines in SW480, HCT116, and the negative in CaCO2, SW620. Considering of PEI and Chitosan. we linked them together and composition,particle size,as well as the zeta potential of CP complex was measured.CCK-8 assay was also used to evaluate their toxicity. Using Chitosan-linked-PEI as the vector, PGL3 and EGFP plasmid transfect into four colon cancer cells after 48 h incubation, and the CD133-related protein expressions were assessed by Western blotting analysis empty control group.Conclusions: The CSC marker expression of CD133+ were significantly different.CD133+ was positive in more than 90% of colon cells, SW480 in 96.97%, HCT116 in 92.96%, however, low expression of CaCO2, SW620 was found in 2.00%, 0.56%.When the W/W of PEI/Chitosan/DNA was 5:4:1, CP complex shows a high transfect efficiency and low toxicity. And when the positive expression of CD133+ was added, the transfect efficiency was decreased. The result of Western blotting analysis showed that the ratios of CD133 in positive group were higher than those in negative group.