Clinical Significance Of Detection And Analysis Of CD4~+CD25~+FOxP3~+ Regulatory T Cells In Peripheral Blood Of Patients With B-cell Lymphoma

Background Non-Hodgkin’s lymphoma( NHL) is a kind of malignant tumour that happens at lymph nodes or extranodal lymphoid tissue. Due to the relationship between it and its immunoregulation as well as the fact that it happens at immune tissue and cells.,it becomes the emphasis of the research on the pathogenesis and the development of malignant lymphoma. Regulatory T cells(Tregs) participate the immunological tolerance process and the mount or functions variation might result in the defect of immunological function, which plays a key role in the immunological tolerance process.and these changes might cause the pathogenesis, development or deteriorate of non-Hodgkin’s lymphoma. The function and structure are based on lots of molecular, including CD4, CD25, CD103, Fox P3 and GITR as well as CTLA-4. Using CD4+CD25+Fox P3+ for the marker to mark regulatory T cells, Tregs’ advantages in specificity and accuracy. However, the emerging data showed controversial result about the function of CD4, CD25 regulatory T cells on the pathogenesis of non-Hodgkin’s lymphoma. Our pre-experiment showed that the concentration of CD4+CD25+Fox P3+ regulatory T cells was up regulated dramatically in B-cell lymphoma when compared with the healthy control subjects. So it is very essential and important for the research to determine the variety of CD4+CD25+Fox P3+ regulatory T cells in B-cell lymphoma patients.Objective: To detect the level of CD4CD25FoxP3 regulatory T cells in the peripheral blood of patients with B-cell lymphoma and the patients with a normal conditions. In this study, we would like to make a preliminarily analysis of the role of CD4+CD25+Fox P3+ regulatory T cells in the prognosis of B-cell lymphoma. We also discuss the relationship between regulatory T cells and the pathogenesis, progress and prognosis of B-cell lymphoma in order to supply references for clinical diagnosis, therapy.Methods: 38 B-cell lymphoma patients and 20 normal controls subjects from April 2013 to December 2013 in our hospital have been adopted in our research. The proportions of CD4+CD25+Fox P3+ regulatory T cells in peripheral blood were detected using flow cytometry before and after chemotherapy. The level changes of CD4+CD25+Fox P3+ regulatory T cells in different patients and different additions among patients are also determined by the same method; Meanwhile, the relativitybetween the CD4+CD25+Fox P3+ T cells of patients with B-cell lymphoma and the prognosis.Results: To the patients with initial certification, the proportion of CD4+CD25+Fox P3+ regulatory T cells was significantly higher than that in healthy controls group, with a significant statistical differences(P<0.05). After chemotherapy the proportion of CD4+CD25+Fox P3+ regulatory T cells was reduced significantly in patients acquired complete or partial remission, with a dramatically statistical differences(P<0.05) However, no significant difference was found between patients acquired complete remission and the patients who acquired partial remission(P> 0.05)..Meanwhile, there are no dramatically statistical differences between CD4+CD25+Fox P3+ regulatory T cells and the pathological types, the IPI index and Ann Arbor stages(P>0.05). There are positive correlations between CD4+CD25+Fox P3+ regulatory T cells and LDH as well asβ2 microglobulin in patients with B cell lymphomaConclusion: CD4+CD25+Fox P3+ regulatory T cells in peripheral blood of B-cell lymphoma patients increase significantly, without any relationship with the clinical stages, the pathological types, the IPI index and Ann Arbor stages of the patients. While there is a decrease in CD4+CD25+Fox P3+ regulatory T cells after the effective chemotherapy, and there are positive correlations between CD4+CD25+Fox P3+ regulatory T cells and LDH as well asβ2 microglobulin. This is impossibly explained, the levels of CD4+CD25+Fox P3+ regulatory T cells in peripheral blood is the main reference index to measure the curative effect of B-cell lymphoma, and the examination on the levels of CD4+CD25+Fox P3+ regulatory T cells in peripheral blood is of clinical value in judging the prognosis of B-cell lymphoma.