Protective Effects Of Schisandrin B On Human Renal Tubular Epithelial Cells With Hypoxia Injury

Objects: To explore the protective effects of schisandrin B(Sch B)on human renal tubular epithelial—HK-2 cells with hypoxia injury induced by cobaltous chloride(Co Cl2), and the possible mechanism thereof.Methods: Cells were randomly assigned to six groups: control group : in which cells were untreated; model group: in which cells were treated with 600 μmol/L Co Cl2 for 24 hours; 1 μmol/L Sch B pretreat group: in which cells were pretreated with 1 μmol/L Sch B for 2 hours,then treated by the same method as model group; 10 μmol/L Sch B pretreat group: in which cells were pretreated with 10 μmol/L Sch B for 2 hours,then treated by the same method as model group; 1 μmol/L Sch B group: cells were treated with 1 μmol/L Sch B for 2 hours; 10 μmol/L Sch B group: cells were treated with 10 μmol/L Sch B for 2 hours. Using CCK-8 kit and flow cytometry to detect the cell viability and apoptosis of HK-2 cells,Western Blot assessed the protein expression of hypoxia-inducible factor 1, alpha subunit(HIF-1α)and P53. The expression of HIF-1α and inducible nitric oxide synthase(i NOS)m RNA was determined by RT-PCR.Results: Compared with the control group, after treated with 600 μmol/L Co Cl2, the cell viability of HK-2 cells was decreased and the apoptosis rate was increased, the expression of HIF-1α protein and i NOS m RNA were up-regulated, the expression of P53 was down-regulated. There was no significant difference between the control group and model group of the expression of HIF-1α m RNA. Compared with the model group, after pretreated with 1 μmol/L and 10 μmol/L Sch B, the cell viability of HK-2 cells was increased and the apoptosis rate was decreased, the expression of HIF-1α and i NOS were down-regulated, the expression of P53 was up-regulated. And 10 μmol/L Sch B was more effective than 1 μmol/L Sch B. There was no significant difference between the control group and the 1 μmol/L and 10 μmol/L Sch B group of the cell viability, apoptosis rate and the expression of P53 protein and i NOS m RNA. And the 1 μmol/L and 10 μmol/L Sch B group almost didn’t express HIF-1α protein.Conclusions: Pretreatment with 1μmol/L and 10μmol/L Sch B can reduce the apoptosis of HK-2 cells by inhibiting the expression of HIF-1α and i NOS, which shows protective effects against hypoxia injury, and 10μmol/L Sch B is more effective than 1μmol/L Sch B. 1μmol/L and 10μmol/L Sch B have no harmful effect on HK-2 cells.