| Objective: Eczema is a kind of skin inflamation with obvious seeps tendency which is caused by a variety of internal and external factors.and eczema have these kinds of features,skin rash diversity,urticant acuteness,easy to break out repeatedly.The pathogenesis of eczema is mainly caused by complex internal and external motivating factor.The study found that the main pathological mechanism is cytokine secretion disorders caused by T helper cells(Th1/Th2) imbalance.Interleukin-18(IL-18) and interleukin-33(IL-33) play an important role in Th1/Th2 balance. The research will find the function of IL-18 m RNA and IL-33 m RNA on the pathogenesis of eczema and the correlation IL-18 m RNA and IL-33 m RNA with related laboratory index.The experiment preliminary discussion: 1. The expression quantity differences of IL-18 m RNA and IL-33 m RNA in peripheral blood single nuclear cell(PBMC) of eczema patients and normals. 2. The expression quantity differences of IL-18 m RNA and IL-33 m RNA in PBMC of different type of eczema patients. 3. The expression quantity differences of IL-18 m RNA and IL-33 m RNA in PBMC of eczema patients after Total Ghcosides of Paconia(TGP)treatment for two weeks and four weeks. 4. The correlation between IL-18 m RNA and IL-33 m RNA,which discuss the interaction of the two factors on the pathogenesis of eczema. 5. The correlation between the expression quantity of IL-18 m RNA and IL-33 m RNA in PBMC of eczema patients and eczema area and severity index(EASI). 6. The correlation between the expression quantity of IL-18 m RNA and IL-33 m RNA and some laboratory index.7.The correlation between the expression quantity of IL-18 m RNA and IL-33 m RNA and family allergies history.Methods: In the study,we chose 80 cases of eczema patients from the affiliated hospital of tianjin traditional Chinese medicine academy and tianjin peoples hospital outpatient service as the experimental observation.According to its history andmanifestations,the 80 cases were dividid into acute,subacute and chronic eczema group,which include 30 cases, 20 cases and 30 cases, respectively. The diagnostic criteria of eczema is on the basis of journal of clinical dermatology whose chief editor is zhaobian. Otherwise, we randomly chose 40 cases as normal control group from the gerneral hospital of tianjin medical university The control group had no special history,recent within one month of no systemic infectious diseases,without use of antibiotics and corticosteroids.The three group is no significance with gender and age distribution.In the experiment,we use Real-time Fluorescence Quantitative Reverse Transcription Polymerase Chain Reaction(RT-PCR) method to analyze the expression quantity of IL-18 m RNA and IL-33 m RNA in PBMC of 80 cases eczema patients and 40 cases normal control.and the amplification of the product by 1.5% agarose gel electrophoresis, under the gel imager camera confirmed.According to the EASI criteria,we calculate the lesion and severity score of 80 cases eczema patients and 40 cases normal control.The statistical software SPSS18.0 was used to process experimental data,with P<0.05 or P<0.01 was statistically significant.Results: IL-18⊿CT in PBM C of eczema group and normal control was 5.60±0.88,6.73±0.35respectively;IL-33⊿CT was 3.04±0.80,6.39±0.29 respectively,all differences were statistically significant(P﹤0.01). 1.IL-18⊿CT in PBM C of acute,subacute,chronic eczema group and normal control was 5.17±0.73,5.37±0.68,6.18±0.85,6.73±0.35 respectively,all differences were statistically significant(P﹤0.01). 2.IL-33⊿CT in PBM C of acute,subacute,chronic eczema group and normal control was 2.72±0.62,2.78±0.45,3.51±0.87,6.39±0.29 respectively,all differences were statistically significant(P﹤0.01). 3.For the first time to see doctor,IL-18 and IL-33⊿CT in PBM C of eczema patients was 5.39±0.83, 2.93±0.86respectively;after treatment for two weeks,IL-18 and IL-33⊿CT was 7.28±0.65,3.23±0.75respectively;after treatment for four weeks,IL-18 and IL-33⊿CT was 8.37±0.77,5.13±0.68 respectively,all differences were statistically significant(P﹤0.01). 4.IL-18 and IL-33⊿CT were positively correlated,r=0. 68, the differences werestatistically significant(P﹤0.01);IL-18 and IL-33⊿CT were negative correlation with EASI,r=-0.62,-0.29 respectively,all differences were statistically significant(P﹤0.01). 5.IL-18 and IL-33⊿CT were negative correlation with the num ber of eosinophi ls, r=-0.56,-0.59 respectively,all differences were statistically significant(P﹤0.01).IL-18 and IL-33⊿CT have no obvious relationship between white blood cells and lymphocyte count(P>0.05). 6.IL-18 and IL-33⊿CT in eczema patients with family allergy history were 5.29±0.77,2.76±0.63 respectively,IL-18 and IL-33⊿CT in no family allergy history patients were 6.36±0.66,3.74±0.74 respectively,all differences were statistically significant(P﹤0.01).Conclusions: The expression quantity of IL-18 and IL-33 in PBMC of eczema patients were higher than healthy controls,and the expression quantity of IL-18 and IL-33 were positive correlation with EASI,these data indicate IL-18 and IL-33 participate in the development of eczema.And the expression quantity of IL-18 and IL-33 were positive correlated with the count of eosinophils,so we can regard eosinophil as a clinical laboratory index for eczema severity.With TGP treatment for two weeks and four weeks,the expression quantity of IL-18 and IL-33 were both decreased,it indicate TGP would be helpful of the treatment of eczema,and can adjust the expression changes of IL-18 and IL-33.The expression quantity of IL-18 and IL-33 in PBMC of eczema patients with a family allergy history were higher than s with no family allergy history group,the result tell us the allergy history may be a risk factor of eczema. |
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