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Effects Of Simulated Microgravity On Explanted Tissues And Cells From Different Regions Of Mammal Brain

Posted on:2017-03-16Degree:MasterType:Thesis
Country:ChinaCandidate:F ZengFull Text:PDF
GTID:2284330503958667Subject:Biology
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Objective: Microgravity is one of the crucial factors in space that affect healthy and work efficiency of astronauts. Plus, numerous study about impacts of microgravity or simulated microgravity on cells, animals, human or so have been carried out till now. To our knowledge, there are few researches focused on the response of different regions of brain to the microgravity environment. However, different brain regions perform different function in organisms and whether they react to microgravity in the same way is still unknown. Therefore, exploring the influence of microgravity on several brain regions is needed. In the present experiments, we investigated whether simulated microgravity could influence morphology and apoptosis of explanted tissues and primary cells from different regions of brain.Methods: Explanted tissues and primary cells were obtained from three regions of neonatal SD rats, namely hippocampus, cerebellum and cortex. After about 7-day culturing at 37℃, 95% of humidity and 5% CO2, cultures were separated into control group(Ctr) and simulated microgravity group(sMG) randomly. The sMG was exposed to clinostat for 1day, 7days and 14 days and control group was cultured on the same horizon with the clinostat. Then, phase contrast microscope and HE staining were occupied to detect the changes of surface morphology and internal morphology. What’s more, scanning electron microscope(SEM) and transmission electron microscope(TEM) were used to find changes of cell morphology and the cytoskeleton. In addition, apoptosis was measured by flow cytometry with the help of Apoptosis Detection Kit. On the top of that, human glioma tissues which were offered by Xuan Wu Hospital were cut into explanted tissue blocks. The procedure was totally the same with that of SD rats and flow cytometry was also employed to detect differences in apoptosis.Results: As shown in the results, after treated with series of time of simulated microgravity, the surface of explanted tissues changed a lot compared to Ctr, especially the edge of tissue blocks became brighter. And the cells within tissue blocks became less and the cytoskeleton underwent changes after 1-day rotating. But apoptosis of primary cells of the three regions showed no difference after 1-day and 7-day rotating, while after 14 days of stimulating by simulated microgravity the apoptosis rate decreased dramatically. At last, data of human glioma cells showed that the situation of apoptosis had similarity with that of SD rats.Conclusions: Simulated microgravity affects the cytoskeleton and morphology of hippocampus, cerebellum and cortex of neonatal SD rats, but has no impact on apoptosis except rotating for a long time. So we conclude that cells can get used to simulated microgravity after a period of time and the changes of the three regions we examined show no difference between each other. Also, using rats as model can imitate the model of human cells when a microgravity experiment carried out.
Keywords/Search Tags:Simulated microgravity, different regions of brain, glioma, morphology structure, apoptosis
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