The Cytotoxic Effects Of Di-(2-ethylhexyl) Phthalate On MIN6 β Cells And Antagonistic Effect Of Vitamin C And Vitamin E

Objective Di-(2-ethylhexyl) phthalate is an endocrine disruptor widely applied by people,ubiquitously found in the environment. It is susceptible to leaching from the matrix, making people widely exposed. Epidemiological and animal studies provided evidence of a significant association between DEHP exposure and adverse glucometabolic outcome. However, the study on cytotoxic effects of DEHP in pancreatic beta-cells is really little. In order to investigate the cytotoxic effects of DEHP on pancreatic beta-cells, we choose MIN6 β cells as the research object.To observe the adverse effects of DEHP on cell viability,cell apoptosis and cell cycle. We also investigate the antagonistic effect of vitamin C and vitamin E on DEHP-induced cytotoxic effects.Therefore, investigate the correlation and mechanism between DEHP exposure and glucose metabolism disorder may provide a theoretical basis for the clinical prevention and treatment of this cytotoxic effects.Method Choose MIN6 β cells as the research object. As treatment group MIN6 β cells were exposed to 0.25, 0.5, 1.0 or 2.0 mM DEHP. Intervention group were exposed to 0.25, 0.5, 1.0or 2.0 mM DEHP and vitamin C(50μM) plus vitamin E(50μM). Cell viability were measured by MTT test after 16, 48 and 72 hours. Apoptosis rate and cell cycle were measured by flow cytometry after 48 hours.Results Compared with the solvent control group(1) With the increase of DEHP concentration and time, cell viability of MIN6 β cells significantly decreased( P < 0.01).(2)Different concentrations of DEHP induced apoptosis in MIN6 β cells(P<0.01).(3) DEHP significantly disrupted cell cycle in MIN6 β cells, it involved in cell cycle arrest, showed a higher number of G0/G1 cells and a reduced percentage of cells entering S phase and M phase(P<0.01).(4) Antioxidant vitamins(C and E) have a protective role against the adverse effect of DEHP on MIN6 β cells.Conclution The results showed that DEHP decreased cell viability, induced apoptosis and disrupted cell cycle in MIN6 β cells. Antioxidant vitamins(C and E) have a protective role againstthe adverse effect of DEHP on MIN6 β cells.